LncRNA CYP1B1-AS1 as a clinical biomarker exacerbates sepsis inflammatory response via targeting miR- 18a- 5p.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:40234801", "kind": "science-record", "revision": 4, "content_hash": "sha256:ccc72314b0bda98a3daae0e73ae8384aad26427d6e8b56d92004b23a565aa3f4", "created_at": "2026-11-01T20:30:24Z", "updated_at": "2026-11-01T22:56:22Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "LncRNA CYP1B1-AS1 as a clinical biomarker exacerbates sepsis inflammatory response via targeting miR- 18a- 5p.", "doi": "10.1186/s12865-025-00712-9", "pmid": "40234801", "authors": ["Lixia Xu", "Jingpo Li", "Li Li", "Qiushuang Zhang", "Qiuju Feng", "Lijie Bai"], "journal": "BMC immunology", "year": 2025, "abstract": "BACKGROUND: Sepsis, characterized by high morbidity and mortality, necessitates the identification of novel diagnostic and prognostic biomarkers to enhance patient outcomes. Prior research has highlighted the potential clinical utility of long non-coding RNAs (lncRNAs) in sepsis. This study aimed to investigate the clinical significance and underlying mechanisms of serum lncRNA Cytochrome P450 family 1 subfamily B member 1 antisense RNA 1 (CYP1B1-AS1) expression in sepsis.\nMETHODS: Differentially expressed lncRNAs in sepsis patients were explored via GEO database. Sepsis patients and Control subjects were included. An in vitro cellular model was established with LPS-stimulated THP- 1 cells. RT-qPCR assessed CYP1B1-AS1 and miR- 18a- 5p expression. ROC analysis evaluated diagnostic and predictive value. Kaplan-Meier curves and Cox regression analyzed the prognostic value of CYP1B1-AS1. Flow cytometry and ELISA assessed cell apoptosis and inflammatory factors levels. Dual luciferase reporter, RIP, and RNA pull down to validate target binding relationship.\nRESULTS: The GSE217700 database shows that CYP1B1-AS1 was upregulated in sepsis. Serum levels of CYP1B1-AS1 were higher in sepsis patients than controls. CYP1B1-AS1 was positively correlated with SOFA and APACHE II scores and distinguished sepsis patients from controls. The 28-day mortality rate for sepsis patients was 29.31%. High CYP1B1-AS1 expression in sepsis patients predicts a worse prognosis and is a potential risk factor. CYP1B1-AS1 targets miR- 18a- 5p. Silencing CYP1B1-AS1 reduced LPS-inducted apoptosis and inflammatory factor promotion, which the miR- 18a- 5p inhibitor reversed.\nCONCLUSION: CYP1B1-AS1 serves as a biomarker for sepsis diagnosis and poor prognosis, potentially promoting inflammation and apoptosis by targeting miR- 18a- 5p.", "claims": [{"id": "claim-method", "statement": "The diagnostic and predictive value of lncRNA was evaluated using ROC curves. Pearson Pearson coefficient was used for correlation analysis, Kaplan-Meier for survival analysis, and Cox regression for survival risk factor analysis.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40234801", "locator": "sections[16] \"Statistical analysis\" paragraph 2", "quote": "The diagnostic and predictive value of lncRNA was evaluated using ROC curves. Pearson Pearson coefficient was used for correlation analysis, Kaplan-Meier for survival analysis, and Cox regression for survival risk factor analysis."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets used and analysed during the current study are available from the corresponding author on reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40234801", "locator": "sections[28] \"Data availability\" paragraph 2", "quote": "The datasets used and analysed during the current study are available from the corresponding author on reasonable request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "CYP1B1-AS1 serves as a biomarker for sepsis diagnosis and poor prognosis, potentially promoting inflammation and apoptosis by targeting miR- 18a- 5p.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:40234801", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "CYP1B1-AS1 serves as a biomarker for sepsis diagnosis and poor prognosis, potentially promoting inflammation and apoptosis by targeting miR- 18a- 5p."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "CYP1B1-AS1 serves as a biomarker for sepsis diagnosis and poor prognosis, potentially promoting inflammation and apoptosis by targeting miR- 18a- 5p.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:40234801"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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