Screening, Analysis, and Validation of Endoplasmic Reticulum Stress-Related DEGs in Epilepsy.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:40413658", "kind": "science-record", "revision": 4, "content_hash": "sha256:293d163f4b6da500f0b50af586cf5d25bff0a6f5509ddfd9413107ebbace6a5c", "created_at": "2026-11-01T20:30:33Z", "updated_at": "2026-11-01T22:56:34Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Screening, Analysis, and Validation of Endoplasmic Reticulum Stress-Related DEGs in Epilepsy.", "doi": "10.1007/s10571-025-01567-9", "pmid": "40413658", "authors": ["Sunyena Shi", "Xu Feng", "Zhan Cao", "Lin Wang", "Mingjian Sun", "Ziyi Zhao", "Wei Sun"], "journal": "Cellular and molecular neurobiology", "year": 2025, "abstract": "To investigate the relationship between Endoplasmic Reticulum Stress (ERS) and epilepsy, as well as their biological functions. We downloaded the GSE143272 dataset from the GEO database, identified differentially expressed genes (DEGs), and cross-analyzed them with ERS-related genes from GeneCards and the Molecular Signatures Database (MSigDB). Protein-protein interaction (PPI) networks were constructed, and Hub genes were screened. ROC curve analysis was conducted to assess the diagnostic utility of these genes, followed by qRT-PCR validation. This study identified a total of 83 ERS-related DEGs in epilepsy. PPI network analysis revealed eight feature genes: C-X-C motif chemokine ligand 8 (CXCL8), Toll-like receptor 4 (TLR4), Matrix metalloproteinase 9 (MMP9), Tumor necrosis factor receptor superfamily member 1A (TNFRSF1A), Prostaglandin-endoperoxide synthase 2 (PTGS2), Signal transducer and activator of transcription 1 (STAT1), B-cell lymphoma 2 (BCL2), and RELA proto-oncogene, NF-κB subunit (RELA). ROC curve analysis demonstrated that the combination of these eight feature genes exhibited the highest diagnostic potential. Among them, CXCL8 was the most valuable gene. qRT-PCR analysis showed that CXCL8 mRNA expression was significantly lower in the case group compared to the control group (P < 0.01). The results suggest that ERS is involved in physiological processes such as inflammation and neuronal apoptosis in epilepsy. This provides a bioinformatics evidence for exploring the biological functions and pathology of ERS in epilepsy, as well as serving as a reference for clinical diagnosis and potential therapeutic targets.", "claims": [{"id": "claim-method", "statement": "In screening for DEGs within the GSE143272 microarray data, we employed the limma package.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40413658", "locator": "sections[4] \"Differential Expression Gene Screening\" paragraph 2", "quote": "In screening for DEGs within the GSE143272 microarray data, we employed the limma package."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets analyzed during the current study are available in the GEO database (https://www.ncbi.nlm.nih.gov/geo)(GSE143272), GeneCards database (https://www.genecards.org/), and MsigDB (https://www.gsea-msigdb.org/gsea/msigdb/index.jsp). Further inquiries can be addressed directly to the corresponding author.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40413658", "locator": "sections[22] \"Data Availability\" paragraph 2", "quote": "The datasets analyzed during the current study are available in the GEO database (https://www.ncbi.nlm.nih.gov/geo)(GSE143272), GeneCards database (https://www.genecards.org/), and MsigDB (https://www.gsea-msigdb.org/gsea/msigdb/index.jsp). 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