PI3K/AKT signaling pathway plays an important role in the pathogenesis of metabolic dysfunction-associated steatotic liver disease.

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Data from three MASLD-related datasets (GSE89632, GSE72756 and GSE49541) were downloaded from the Gene Expression Omnibus (GEO) database and merged for analysis. Differentially expressed genes (DEGs) were identified via the limma package in R (|logFC|> 1, adjusted p value < 0.05). Functional enrichment analysis was conducted via Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways and gene set enrichment analysis (GSEA). Protein‒protein interaction (PPI) network analysis was used to identify hub genes related to MASLD. 1278 DEGs (1238 upregulated, 40 downregulated) related to MASLD were identified. GO analysis revealed that the DEGs were involved mainly in the regulation of membrane potential (BP), the monoatomic ion channel complex (CC) and postsynaptic neurotransmitter receptor activity (MF). KEGG analysis highlighted neuroactive ligand-receptor interactions and taste transduction pathways, which were downregulated in MASLD. PPI network analysis identified 10 hub genes: PIK3CD, PIK3R2, PIK3R1, PIK3R3, PIK3CB, PIK3CA, SRC, PIK3CG, PIK3R5 and PIK3R6. This study identified 10 hub genes associated with MASLD, primarily involved in the PI3K/AKT signaling pathway, which could serve as biomarkers for MASLD diagnosis and progression, with the pathway potentially becoming a new therapeutic target.", "claims": [{"id": "claim-method", "statement": "The limma package in R was used to identify DEGs between the MASLD samples and HC group samples in the combined dataset.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40594616", "locator": "sections[4] \"Identifcation of differential expressed genes (DEGs) associated with MASLD\" paragraph 2", "quote": "The limma package in R was used to identify DEGs between the MASLD samples and HC group samples in the combined dataset."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Three MASLD-related datasets (GSE89632 series deposited by Allard JP et al., GSE72756 series deposited by Chuanzheng S et al. and GSE49541 series deposited by Caba O et al.) were downloaded from the Gene Expression Omnibus (GEO) database in NCBI (http://www.ncbi.nlm.nih.gov/gds/).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40594616", "locator": "sections[3] \"Data acquisition and preliminary processing\" paragraph 2", "quote": "Three MASLD-related datasets (GSE89632 series deposited by Allard JP et al., GSE72756 series deposited by Chuanzheng S et al. and GSE49541 series deposited by Caba O et al.) were downloaded from the Gene Expression Omnibus (GEO) database in NCBI (http://www.ncbi.nlm.nih.gov/gds/)."}], "state": "complete"}, {"id": "claim-target-result", "statement": "This study identified 10 hub genes associated with MASLD, primarily involved in the PI3K/AKT signaling pathway, which could serve as biomarkers for MASLD diagnosis and progression, with the pathway potentially becoming a new therapeutic target.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:40594616", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "This study identified 10 hub genes associated with MASLD, primarily involved in the PI3K/AKT signaling pathway, which could serve as biomarkers for MASLD diagnosis and progression, with the pathway potentially becoming a new therapeutic target."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "This study identified 10 hub genes associated with MASLD, primarily involved in the PI3K/AKT signaling pathway, which could serve as biomarkers for MASLD diagnosis and progression, with the pathway potentially becoming a new therapeutic target.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:40594616"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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