Identification of key genes as diagnostic biomarkers for IBD using bioinformatics and machine learning.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:40611294", "kind": "science-record", "revision": 3, "content_hash": "sha256:bfbf990b8b7f01e0df7f7612b2430d0573babbadc3edf4a4ff58cf0416f84f00", "created_at": "2026-11-01T20:30:42Z", "updated_at": "2026-11-01T22:14:18Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Identification of key genes as diagnostic biomarkers for IBD using bioinformatics and machine learning.", "doi": "10.1186/s12967-025-06531-1", "pmid": "40611294", "authors": ["Tianhao Li", "Haoren Jing", "Xinyu Gao", "Te Zhang", "Haitao Yao", "Xipeng Zhang", "Mingqing Zhang"], "journal": "Journal of translational medicine", "year": 2025, "abstract": "BACKGROUND: The pathogenesis of inflammatory bowel disease (IBD) involves complex molecular mechanisms, and achieving clinical remission remains challenging. This study aims to identify IBD-potential biomarkers, analyze their correlation with immune cell infiltration, and identify genes that have a causal relationship with IBD.\nMETHODS: RNA-seq datasets for IBD were retrieved from GEO, stratified into discovery (GSE75214), validation (GSE36807), and independent testing cohorts (GSE179285, GSE47908). Through comparative expression profiling of the discovery cohort, IBD-associated differentially expressed genes (DEGs) were detected. Core candidate genes were subsequently prioritized using protein-protein interaction network analysis, further refined through machine learning approaches (Random Forest/Support Vector Machines). Immune cell abundance quantification and statistical correlation analyses with IBD-associated transcripts were conducted via the CIBERSORTx deconvolution algorithm. To complement these findings, blood expression quantitative trait loci (eQTL) data from GTExv8.ALL.Whole_Blood were integrated with IBD genome-wide association statistics from the FinnGen consortium. This multi-omics integration framework employed: (1) Bayesian colocalization to assess shared causal variants, (2) HEIDI heterogeneity testing, and (3) summary Mendelian randomization (SMR) for causal inference validation.\nRESULTS: Three genes, IRF1, GBP5, and PARP9, demonstrated significant IBD-promoting effects. The characteristic biomarkers of IBD were associated with immune cell infiltration. SMR analysis based on eQTL data showed that IRF1 was significantly associated with the risk of IBD. Moreover, IRF1 passed the HEIDI test of > 0.05 on gene expression, and IRF1 demonstrated the ability to promote the development of IBD.\nCONCLUSIONS: This integrative analysis identifies IRF1, GBP5, and PARP9 as potential genes associated with IBD pathogenesis. SMR analysis based on eQTL data revealed that IRF1 was significantly associated with the risk of IBD. The relationship between IRF1 and IBD risk highlights its potential as both a therapeutic target and diagnostic biomarker.", "claims": [{"id": "claim-method", "statement": "This multi-omics integration framework employed: (1) Bayesian colocalization to assess shared causal variants, (2) HEIDI heterogeneity testing, and (3) summary Mendelian randomization (SMR) for causal inference validation.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40611294", "locator": "sections[0] \"Abstract\" paragraph 2", "quote": "This multi-omics integration framework employed: (1) Bayesian colocalization to assess shared causal variants, (2) HEIDI heterogeneity testing, and (3) summary Mendelian randomization (SMR) for causal inference validation."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40611294", "locator": "sections[31] \"Data availability\" paragraph 2", "quote": "The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request."}], "state": "complete"}, {"id": "claim-target-result", "statement": "SMR analysis based on eQTL data revealed that IRF1 was significantly associated with the risk of IBD.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:40611294", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "SMR analysis based on eQTL data revealed that IRF1 was significantly associated with the risk of IBD."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "SMR analysis based on eQTL data revealed that IRF1 was significantly associated with the risk of IBD.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:40611294"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE179285"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE179285"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:40611294", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.1186/s12967-025-06531-1", "sha256": 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