Identification of potential miRNA biomarkers for neurobrucellosis diagnosis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:40626048", "kind": "science-record", "revision": 3, "content_hash": "sha256:ef4a69debd9a63c1ad59d6eea68561e42b221a1a09ccc5c24c126c777c6617c9", "created_at": "2026-11-01T20:30:43Z", "updated_at": "2026-11-01T22:14:19Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Identification of potential miRNA biomarkers for neurobrucellosis diagnosis.", "doi": "10.3389/fphys.2025.1463597", "pmid": "40626048", "authors": ["Meiling Liu", "Liping Feng", "Shujun Shi", "Yuzhou Guo", "Menghan Lv", "Zhengyu Li", "Xintian Wang", "Hao Yang", "Zhelin Zhang"], "journal": "Frontiers in physiology", "year": 2025, "abstract": "Background: Neurobrucellosis (NB) is a rare complication of brucellosis that presentis with a variety of clinical symptoms, complicating its diagnosis. This study aimed to analyze the microRNA (miRNA) profile of cerebrospinal fluid (CSF) in NB patients to identify potential biomarkers for NB diagnosis.\nMethods: Small RNA sequencing was utilized to identify differential expressed miRNAs (DEmiRNAs) in CSF samples from both control and NB patients (in-house cohort). The GSE107554 dataset (validation cohort) from the GEO database was used to validate the expression levels of these DEmiRNAs. Subsequently, LASSO regression analysis was conducted to construct a diagnostic risk score model based on common miRNAs identified in both the in-house and validation cohorts.\nResults: The study identified 51 DEmiRNAs between the control and NB groups, based on the data from the in-house cohort. Among these, four common miRNAs (miR-342-3p, miR-576-5p, miR-15b-5p and miR-499a-5p) were significantly elevated in the infection group compared to controls in both the in-house and validation cohorts. Subsequently, a novel NB diagnostic signature comprising two miRNAs (miR-576-5p and miR-499a-5p) was developed using the LASSO regression analysis. Receiver operating characteristic (ROC) analysis demonstrated the good diagnostic potential of this miRNA signature in both cohorts. RT-qPCR analysis indicated elevated levels of miR-576-5p and miR-499a-5p in CSF samples of NB patients compared to controls. Additionally, RT-qPCR results revealed decreased levels of VAV3 (vav guanine nucleotide exchange factor 3, a target of miR-499a-5p) and IGF1 (insulin like growth factor 1, a target of miR-576-5p) in the CSF samples of NB patients.\nConclusion: Collectively, miR-576-5p and miR-499a-5p may serve as potential biomarkers for the diagnosis of NB.", "claims": [{"id": "claim-method", "statement": "LASSO Cox regression analysis with was conducted using the “glmnet” package in R language to further identify miRNAs associated with NB (Friedman et al., 2010).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40626048", "locator": "sections[8] \"2.6 LASSO regression analysis\" paragraph 2", "quote": "LASSO Cox regression analysis with was conducted using the “glmnet” package in R language to further identify miRNAs associated with NB (Friedman et al., 2010)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data supporting the conclusions of this study are openly available at https://www.ncbi.nlm.nih.gov/search/all/?term=PRJNA1272809, reference number PRJNA1272809.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40626048", "locator": "sections[22] \"Data availability statement\" paragraph 2", "quote": "Data supporting the conclusions of this study are openly available at https://www.ncbi.nlm.nih.gov/search/all/?term=PRJNA1272809, reference number PRJNA1272809."}], "state": "complete"}, {"id": "claim-target-result", "statement": "A two-miRNA diagnostic signature comprising miR-576-5p and miR-499a-5p showed strong diagnostic performance for neurobrucellosis, with ROC AUC values of 1.000 in the in-house cohort and 0.875 in the validation cohort.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:40626048", "locator": "sections[16] \"3.3 Construction a diagnostic risk score model\" paragraph 2", "quote": "As shown in Figures 4B, C, the AUC value of the ROC curve was 1.000 in the in-house cohort, and was 0.875 in the validation cohort, indicating that the risk score may have a good diagnostic performance."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "A two-miRNA diagnostic signature comprising miR-576-5p and miR-499a-5p showed strong diagnostic performance for neurobrucellosis, with ROC AUC values of 1.000 in the in-house cohort and 0.875 in the validation cohort.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:40626048"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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Methods: Small RNA sequencing was utilized to identify differential expres", "patients in the NB group were used for small RNA-sequencing. Subsequently, the CSF samples from 15 patie"], "order_basis": "paper_text_order:352", "id": "assay:1"}, {"role": "data_generation", "definition_id": "qpcr", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["ial of this miRNA signature in both cohorts. RT-qPCR analysis indicated elevated levels of miR-57", "patients compared to controls. Additionally, RT-qPCR results revealed decreased levels of VAV3 (v", "of this miRNA signature in both cohorts. RT-qPCR analysis indicated elevated levels of miR-57", "ients compared to controls. Additionally, RT-qPCR results revealed decreased levels of VAV3 (v"], "order_basis": "paper_text_order:1406", "id": "assay:2"}, {"role": "analysis", "definition_id": "survival_analysis", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": ["cox_ph"], "support_state": "supported", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["LASSO Cox regression analysis with was conducted using the “glmnet” package in R language to further identify miRNAs associated with NB (Friedman et al., 2010)."], "order_basis": "consumes_earlier_output", "id": "assay:3"}]}