MTHFD1L is a novel prognostic marker and therapeutic target in cutaneous melanoma.

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"source_hash": "e8bcccc78ba07f9beab001dd8ee5fe95c3910429887bbdb31d33d6468b17e5c0", "raw_response": "{\"parent_value\":0,\"unit\":\"\",\"evidence\":\"\",\"target_result\":\"The paper states that MTHFD1L expression was increased in SKCM tissues, high MTHFD1L expression correlated with poor overall survival, and MTHFD1L knockdown significantly inhibited proliferation, migration, invasion, and clonogenic ability in melanoma cells; no numerical result is stated for the main finding.\",\"number_stated\":false}", "reading": {"parent_value": 0, "unit": "", "evidence": "", "target_result": "The paper states that MTHFD1L expression was increased in SKCM tissues, high MTHFD1L expression correlated with poor overall survival, and MTHFD1L knockdown significantly inhibited proliferation, migration, invasion, and clonogenic ability in melanoma cells; no numerical result is stated for the main finding.", "number_stated": false, "source_locator": null}, "recorded_at": "2026-10-31T15:19:52.819584+00:00"}, {"provider": "minimax_alt", "model": "MiniMax-M3", "prompt_version": "replication-target-v2", "prompt_hash": "c0c70517422ef012559b13cc0153b3a5b035c3254efa864d9886e4b6203660b3", "source_hash": "e8bcccc78ba07f9beab001dd8ee5fe95c3910429887bbdb31d33d6468b17e5c0", "raw_response": "<think>We need answer JSON only. Need inspect source for single numerical headline result stated for main finding registered target. Text seems abstract/results but no numerical result except sample count 340, 15 nodes, 22 immune cells, threshold 0.900, versions, datasets IDs. Headline main finding perhaps high expression poor OS, no numeric HR given. User explicitly if no number for registered target set false parent 0 unit/evidence empty target_result what text states instead. Need decide perhaps target is \"headline numerical result paper states for main finding\". Source doesn't give numerical value for main finding, likely no number. Need exact schema. target_result say paper states MTHFD1L levels increased and high expression correlated with poor overall survival, knockdown inhibited functions. Since evidence empty. number false. Could there be a number implicitly attached, e.g. TCGA including 340 tumor samples, but not headline result. Registered target is generic main finding; no explicit numerical result. Answer JSON.\n\nNeed ensure valid JSON. target_result concise. \"The paper states that MTHFD1L expression was elevated in SKCM and that high MTHFD1L expression correlated with poor overall survival, but it gives no numerical result for this main finding.\" Unit empty. parent 0. evidence \"\".</think>\n\n{\"parent_value\":0,\"unit\":\"\",\"evidence\":\"\",\"target_result\":\"The paper states that MTHFD1L expression was elevated in SKCM and that high MTHFD1L expression correlated with poor overall survival, but it provides no numerical result for this main finding.\",\"number_stated\":false}", "reading": {"parent_value": 0, "unit": "", "evidence": "", "target_result": "The paper states that MTHFD1L expression was elevated in SKCM and that high MTHFD1L expression correlated with poor overall survival, but it provides no numerical result for this main finding.", "number_stated": false, "source_locator": null}, "recorded_at": "2026-10-31T15:19:55.206953+00:00"}], "agreement": true, "disagreements": {"target_result": {"minimax": "High MTHFD1L expression correlated with poor overall survival in skin cutaneous melanoma.", "minimax_alt": "unclear — the supplied text gives no headline numerical result; it states only that high MTHFD1L expression correlated with poor overall survival.", "decisive": false}, "physical_methods": {"minimax": ["RT-qPCR", "Western blot", "Immunohistochemistry (IHC) staining", "MTHFD1L knockdown in A375 and A2058 melanoma cells", "Proliferation, migration, invasion, and clonogenic ability assays", "Western blot analysis of TGF-β1, SMAD3, and phosphorylated SMAD3"], "minimax_alt": ["RT-qPCR", "Western blot", "IHC staining", "Establishment of MTHFD1L knockdown models in A375 and A2058 melanoma cells", "Functional characterization of proliferation, migration, invasion, and clonogenic ability"], "decisive": false}, "analysis_methods": {"minimax": ["TCGA-SKCM and GTEx mRNA expression analysis", "GSE15605 and GSE19234 gene-expression analysis", "GO enrichment analysis", "KEGG pathway enrichment analysis", "Protein-protein interaction network construction", "GEPIA2 overall-survival analysis using median MTHFD1L expression as the cutoff", "Univariate Cox proportional hazards regression", "Multivariate Cox proportional hazards regression", "Kaplan–Meier survival analysis with log-rank tests", "χ2 test", "Fisher's exact test", "Unpaired t-test", "Two-way analysis of variance (ANOVA)", "CIBERSORT immune-cell infiltration analysis", "xCell immune-cell infiltration analysis", "Spearman correlation analysis"], "minimax_alt": ["TCGA-SKCM and combined GSE15605/GSE19234 analysis of MTHFD1L mRNA expression", "GEPIA2 prognostic analysis", "GO enrichment analysis", "KEGG pathway enrichment analysis", "STRING protein-protein interaction network construction", "CIBERSORT immune-cell infiltration analysis", "xCell immune-cell infiltration analysis", "Kaplan–Meier method with log-rank tests", "Univariate and multivariate Cox proportional hazard regression", "χ2 test", "Fisher’s exact test", "Unpaired t-test", "Two-way analysis of variance (ANOVA)", "Spearman correlation analysis"], "decisive": false}}, "agreement_basis": {"policy": "decisive_fields", "decisive_fields": ["work_type", "number_stated", "parent_value", "unit"]}, "blinded": true}, "human_critique": {"status": "not_run", "reviewer": null, "signed_at": null, "conflict_checked": false, "commentary": null}, "stages": [{"key": "target_materials", "status": "pending", "reason": null, "evidence": null}, {"key": "independent_readings", "status": "passed", "reason": "The independent readings are incomplete or disagree; 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