Identification and analysis of cuproptosis associated molecular clusters and immunological profiles in atopic dermatitis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:40655146", "kind": "science-record", "revision": 3, "content_hash": "sha256:f806c96d980c20f0ce2a5590166552d52f7bf2f1ece4e765e98d01cf6cc53813", "created_at": "2026-11-01T20:30:45Z", "updated_at": "2026-11-01T22:14:21Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Identification and analysis of cuproptosis associated molecular clusters and immunological profiles in atopic dermatitis.", "doi": "10.3389/fimmu.2025.1545457", "pmid": "40655146", "authors": ["Liangzhe Wang", "Bo Wang", "Min Peng", "Xiaoyan Yang", "Sijia Huang", "Ruixin Wang", "Lin Du", "Ruiqian Yao", "Wei Wang", "Baiping Dong", "Yuanjie Zhu"], "journal": "Frontiers in immunology", "year": 2025, "abstract": "Background: Atopic dermatitis (AD) is a chronic skin condition marked by persistent itching and dryness. The role of cuproptosis, a novel form of programmed cell death, in AD is not yet understood.\nMethods: The GSE107361 dataset was obtained from the Gene Expression Omnibus (GEO) database. Cuproptosis-related genes (CRGs) in AD were identified and analyzed, and immune landscape analysis was performed using ssGSEA. AD was clustered based on CRGs using ConsensusClusterPlus. Weighted gene co-expression network analysis (WGCNA) and differential gene expression analysis were conducted. Hub genes between AD clusters were identified, and both protein-protein interaction (PPI) and drug-gene interaction networks were developed.\nResults: Three CRGs (DLD, MTF1, and GLS) were significantly upregulated in the AD group compared to healthy controls. Notably, four core CRGs (LIAS, LIPT1, PDHA1, CDKN2A) distinguished early-onset from adult-onset AD, indicating more active cuproptosis in early-onset AD. CRGs were linked to immune cell infiltration in AD, highlighting differences in immune microenvironments between early- and adult-onset AD. Early-onset AD showed high innate immunity, while adult-onset AD had a mix of innate and type 1 adaptive immunity. CRG expression identified two molecular subtypes with distinct immune infiltration: Cluster 2 (high cuproptosis) had predominant innate immunity, while Cluster 1 (low cuproptosis) had adaptive immunity. Additionally, 102 hub DEGs were identified through WGCNA co-expression network analysis, and 10 hub node genes were identified and potential drugs were explored for the management of AD.\nConclusions: The study provides insights into the roles of cuproptosis-related processes in the pathogenesis and potential treatment of AD. 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