The cardiac phospho-proteome during pressure overload in mice.

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Despite its high prevalence during aortic stenosis or chronic arterial hypertension, the global alterations in cardiac phospho-proteome dynamics following TAC remain incompletely characterised. We present a database of the phospho-proteomic signature one day and seven days after TAC. Utilising proteomic and phospho-proteomic analyses, we quantified thousands of proteins and phosphorylation sites, revealing hundreds of differential phosphorylation events significantly altered in the cardiac response to pressure overload. Our analysis highlights significant changes in hypertrophic signalling, metabolic remodelling, contractile function, and the stress response pathways. We present proteomic data from the main cardiac cell types (endothelial cells, fibroblasts and cardiomyocytes) to reveal the cellular localisation of the detected phospho-proteins, offering insights into temporal and site-specific phosphorylation events, facilitating the potential discovery of novel therapeutic targets and biomarkers. By making this resource publicly available (ProteomeXchange with identifier PXD061784) we aim to enable further exploration of the molecular basis of cardiac remodelling and advance translational research in heart failure.", "claims": [{"id": "claim-method", "statement": "Differential expression was tested using the limma package.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40753091", "locator": "sections[13] \"Data analysis\" paragraph 4", "quote": "Differential expression was tested using the limma package."}], "state": "complete"}, {"id": "claim-data-access", "statement": "No new code was generated in this work.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40753091", "locator": "sections[19] \"Code availability\" paragraph 2", "quote": "No new code was generated in this work."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The study quantified thousands of cardiac proteins and phosphorylation sites and identified hundreds of phosphorylation events significantly altered one day and seven days after TAC.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:40753091", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Utilising proteomic and phospho-proteomic analyses, we quantified thousands of proteins and phosphorylation sites, revealing hundreds of differential phosphorylation events significantly altered in the cardiac response to pressure overload."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "The study quantified thousands of cardiac proteins and phosphorylation sites and identified hundreds of phosphorylation events significantly altered one day and seven days after TAC.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:40753091"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. 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