Exploring molecular links between obesity and osteoporosis: insights from in-silico analysis and mannose supplementation.

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Obesity, a known risk factor for osteoporosis (OP), suggests shared molecular pathways between these conditions. This study explores the molecular mechanisms of obesity-induced OP and the potential therapeutic role of mannose supplementation. Using in-silico analysis, GEO2R was applied to dataset GSE110796 to identify differentially expressed genes (DEGs) under high-fat diet-induced obesity and mannose supplementation. Enrichment analysis via Enrichr and ClueGO revealed significant molecular pathways altered by high-fat diets and reversed by mannose. Similarly, pathways for OP were identified using the GEO dataset GSE56815 and DisGeNET-associated genes. Forty-four overlapping pathways were identified between obesity and OP, with key immune and inflammatory pathways modulated by mannose. Notably, genes upregulated in osteoclast differentiation during obesity were downregulated with mannose. These findings suggest that mannose modulates shared pathways in obesity and OP, offering a cost-effective therapeutic approach. This study enhances understanding of obesity-induced OP and provides a foundation for innovative medical interventions.", "claims": [{"id": "claim-method", "statement": "Expression data from the GSE110796 dataset (platform GPL6887) were analysed using GEO2R33 to identify DEGs in mice fed an HFD or HFD with mannose supplementation. DEGs were filtered based on fold change and a p-value threshold of ≤ 0.05, separately identifying upregulated and downregulated genes.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40789846", "locator": "sections[5] \"Differential gene expression analysis using GEO2R\" paragraph 2", "quote": "Expression data from the GSE110796 dataset (platform GPL6887) were analysed using GEO2R33 to identify DEGs in mice fed an HFD or HFD with mannose supplementation. DEGs were filtered based on fold change and a p-value threshold of ≤ 0.05, separately identifying upregulated and downregulated genes."}], "state": "complete"}, {"id": "claim-data-access", "statement": "Data is provided within the manuscript or supplementary information files.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40789846", "locator": "sections[30] \"Data availability\" paragraph 2", "quote": "Data is provided within the manuscript or supplementary information files."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Forty-four overlapping pathways were identified between obesity and OP, with key immune and inflammatory pathways modulated by mannose.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:40789846", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Forty-four overlapping pathways were identified between obesity and OP, with key immune and inflammatory pathways modulated by mannose."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Forty-four overlapping pathways were identified between obesity and OP, with key immune and inflammatory pathways modulated by mannose.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:40789846"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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