Regulatory role of LncRNA FMR1-AS1 in the pathogenesis of alzheimer's disease based on bioinformatics and in vitro experimental validation.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:40820106", "kind": "science-record", "revision": 4, "content_hash": "sha256:dfdc5be8a4ddef38c3df569603865c331573f69769591a805fde1bf3699438ab", "created_at": "2026-11-01T20:30:54Z", "updated_at": "2026-11-01T22:57:01Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Regulatory role of LncRNA FMR1-AS1 in the pathogenesis of alzheimer's disease based on bioinformatics and in vitro experimental validation.", "doi": "10.1038/s41598-025-15242-y", "pmid": "40820106", "authors": ["Xiang Yu", "Hao Cai", "Cai He", "Zhong Ouyang", "Yongchang Li", "Liang Chen"], "journal": "Scientific reports", "year": 2025, "abstract": "Alzheimer's disease (AD) is a major cause of dementia, characterized by β-amyloid (Aβ) plaque accumulation and Tau protein hyperphosphorylation. Although long non-coding RNAs (lncRNAs) have been implicated in neurodegenerative diseases, their roles in AD remain unclear. This study analyzed RNA sequencing data from the brain tissues of 17 AD patients and 19 healthy controls (GEO: GSE138260) to construct a gene co-expression network and identified eight lncRNAs strongly associated with AD. FMR1-AS1 was selected for functional validation. In an Aβ1-42-induced SH-SY5Y neuronal injury model, overexpression of FMR1-AS1 significantly increased cell viability ([Formula: see text]), inhibited apoptosis ([Formula: see text]), and reduced Tau hyperphosphorylation ([Formula: see text]). FMR1-AS1 also alleviated oxidative stress by lowering reactive oxygen species (ROS) levels ([Formula: see text]), enhanced superoxide dismutase (SOD) activity ([Formula: see text]), and decreased malondialdehyde (MDA) content ([Formula: see text]). Knockdown of FMR1-AS1 exacerbated neuronal damage. These results demonstrate that FMR1-AS1 exerts neuroprotective effects by regulating apoptosis, oxidative stress, and Tau pathology. The study highlights FMR1-AS1 as a potential therapeutic target for AD and may advance the understanding of lncRNA-mediated regulatory mechanisms in neurodegeneration.", "claims": [{"id": "claim-method", "statement": "One-way analysis of variance (ANOVA) was used to evaluate differences among multiple groups, followed by Tukey’s or Dunnett’s post hoc multiple comparisons test. For comparisons between two groups, an unpaired two-tailed Student’s t-test was applied.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40820106", "locator": "sections[17] \"Statistical analysis\" paragraph 2", "quote": "One-way analysis of variance (ANOVA) was used to evaluate differences among multiple groups, followed by Tukey’s or Dunnett’s post hoc multiple comparisons test. 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This study analyzed RNA sequencing data from the brain tissues of 17 AD patient", "lysis, and experimental validation. Based on transcriptomic data from the Gene Expression Omnibus (GEO)"], "order_basis": "paper_text_order:386", "id": "assay:1"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["functional validation. In an Aβ1-42-induced SH-SY5Y neuronal injury model, overexpression of FMR", "Y27 (SNL-092, Sean Biotechnology, China) was cultured in DMEM/F12 medium (12660-012, Gibco) supplemen", "51). To establish an in vitro AD cell model, SH-SY5Y cells were treated with A\\documentclass[12pt", "\\end{document}1-42 treatment on apoptosis in SH-SY5Y cells, flow cytometry was performed using an"], "order_basis": "paper_text_order:659", "id": "assay:2"}, {"role": "data_generation", "definition_id": "apoptosis_assay", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["l viability ([Formula: see text]), inhibited apoptosis ([Formula: see text]), and reduced Tau hyper", "exerts neuroprotective effects by regulating apoptosis, oxidative stress, and Tau pathology. The st", "ts regulatory effects on neuronal viability, apoptosis, Tau protein phosphorylation, and oxidative", "aggregation. Flow cytometry analysis of cell apoptosis To evaluate the effect of A\\documentclass[12"], "order_basis": "paper_text_order:788", "id": "assay:3"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["ldehyde (MDA) content ([Formula: see text]). Knockdown of FMR1-AS1 exacerbated neuronal damage. The", "48-F, Sino Biological Inc., Beijing, China). Transfected cells were harvested and centrifuged at 300"], "order_basis": "paper_text_order:1130", "id": "assay:4"}, {"role": "data_generation", "definition_id": "qpcr", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["tion Quantitative Polymerase Chain Reaction (RT-qPCR), Western blotting, MTT assay, TUNEL stainin", "n Quantitative Polymerase Chain Reaction (RT-qPCR), Western blotting, MTT assay, TUNEL stainin", "8813, Applied Biosystems, USA). Quantitative real-time PCR was performed using SYBR® Green PCR Master M", "ctions were conducted on a CFX Opus Deepwell Real-Time PCR System with the following cycling conditions"], "order_basis": "paper_text_order:2888", "id": "assay:5"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["itative Polymerase Chain Reaction (RT-qPCR), Western blotting, MTT assay, TUNEL staining, flow cytometry,", "ly and late apoptotic cells were quantified. Western blot analysis of Tau protein expression To invest", "of FMR1-AS1 on Tau protein phosphorylation, Western blot analysis was performed. Total proteins were"], "order_basis": "paper_text_order:2898", "id": "assay:6"}, {"role": "data_generation", "definition_id": "flow_cytometry", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["Western blotting, MTT assay, TUNEL staining, flow cytometry, and measurements of ROS, SOD, and MDA level", "ocument}$$\\beta$$\\end{document} aggregation. Flow cytometry analysis of cell apoptosis To evaluate the e", "-42 treatment on apoptosis in SH-SY5Y cells, flow cytometry was performed using an Annexin V-FITC/PI dou"], "order_basis": "paper_text_order:2943", "id": "assay:7"}, {"role": "data_generation", "definition_id": "animal_model", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.69, "evidence": ["i-phospho-Tau (ab109390, 1:1000, Abcam, UK), mouse anti-Tau (ab80579, 1:1000, Abcam, UK), and r"], "order_basis": "paper_text_order:9405", "id": "assay:8"}, {"role": "analysis", "definition_id": "group_comparison", "definition_version": "f2132e96ae6c7a4e", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"dsl_catalogue": "f2132e96ae6c7a4e"}, "confidence": 1.0, "evidence": ["One-way analysis of variance (ANOVA) was used to evaluate differences among multiple groups, followed by Tukey’s or Dunnett’s post hoc multiple comparisons test. 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