Early proteomic response in thyroid gland after 131I administration in female Balb/c mice.

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The aim was to investigate early changes in protein expression in thyroid and plasma from mice injected with 131I as iodide. Female Balb/c nude mice were i.v. injected with 0 or 490 kBq 131I and killed after 24 h. Thyroid and blood samples were collected from each animal. Protein levels were determined by mass spectrometry. Data are available via ProteomeXchange with identifier PXD062861. Altogether, 17 and 20 proteins showed statistically significant altered levels in thyroid gland and plasma, respectively, after 131I exposure. Most of these proteins had decreased and increased levels in thyroid and plasma, respectively. Few of them were previously proposed radiation responsive proteins. Functional annotation suggests impact on haematopoiesis, reduced oxygen levels, and hypothyroidism. The role of CHIA and PGAM2 in radiation-induced response should be further examined, together with identification and validation of biomarkers of 131I exposure.", "claims": [{"id": "claim-method", "statement": "Statistical significance between treated and control group was determined using a Welch’s t-test.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40875264", "locator": "sections[10] \"Database search for protein TMT quantification\" paragraph 2", "quote": "Statistical significance between treated and control group was determined using a Welch’s t-test."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE [28] partner repository with the dataset identifier PXD062861.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40875264", "locator": "sections[10] \"Database search for protein TMT quantification\" paragraph 3", "quote": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE [28] partner repository with the dataset identifier PXD062861."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Altogether, 17 and 20 proteins showed statistically significant altered levels in thyroid gland and plasma, respectively, after 131I exposure.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:40875264", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Altogether, 17 and 20 proteins showed statistically significant altered levels in thyroid gland and plasma, respectively, after 131I exposure."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "Altogether, 17 and 20 proteins showed statistically significant altered levels in thyroid gland and plasma, respectively, after 131I exposure.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:40875264"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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