The ceRNA Regulatory Network in Vitiligo: Evidence from Bioinformatics Analysis.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:40910032", "kind": "science-record", "revision": 3, "content_hash": "sha256:47c86fc5fb158b54bbcac15f60df8d95f600f1850f7ee57706524e526660f795", "created_at": "2026-11-01T20:31:01Z", "updated_at": "2026-11-01T22:14:41Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "The ceRNA Regulatory Network in Vitiligo: Evidence from Bioinformatics Analysis.", "doi": "10.2147/CCID.S528604", "pmid": "40910032", "authors": ["Hedan Yang", "Xiuzhen Li", "Xiaoli Zhang", "Yiping Ge", "Yin Yang", "Tong Lin"], "journal": "Clinical, cosmetic and investigational dermatology", "year": 2025, "abstract": "Background: Vitiligo is an acquired depigmentary disorder caused by the loss of functional melanocytes. Increasing evidence suggests that competing endogenous RNA (ceRNA) interactions participate in this process, yet their global architecture in vitiligo remains unclear.\nObjective: To delineate a long non-coding RNA (lncRNA)-microRNA (miRNA)-mRNA ceRNA network associated with vitiligo and to identify blood-borne RNA markers with diagnostic potential.\nMethods: miRNA, mRNA, and lncRNA expression data from vitiligo patients and healthy controls were obtained from the GEO database (GSE141655 and GSE186928). Differentially expressed (DE) mRNAs, miRNAs and lncRNAs were screened (|log2 FC| > 0.5, adj. p< 0.05). Functional enrichment, STRING-based protein-protein interaction (PPI) mapping, and lncRNA-mRNA co-expression analysis (Pearson r > 0.9) was performed. miRNA-mRNA pairs were predicted with miRWalk 3.0, and miRNA-lncRNA pairs with miRanda v3.3a (score ≥ 140, energy ≤-20 kcal mol-¹). Triplets that shared the same miRNA, displayed positive lncRNA--mRNA correlation, and showed inverse expression relative to the miRNA were combined into a ceRNA network; hub nodes were ranked by degree centrality. Candidate lncRNAs were validated by RT-qPCR in peripheral blood from 20 vitiligo patients and 20 matched controls.\nResults: A total of 454 DE-mRNAs (341 down-, 113 up-regulated), 22 DE-miRNAs (6 down-, 16 up-regulated), and 281 DE-lncRNAs (112 down-, 169 up-regulated) were identified. Enrichment analysis highlighted pathways linked to melanogenesis, oxidative stress, PI3K-Akt, JAK-STAT and IL-17 signalling. The ceRNA network comprised 33 lncRNAs, 12 miRNAs and 58 mRNAs; SLC32A1, GRIA2, PRKACG and WNT1 were top hub proteins in the PPI sub-network. Blood validation confirmed up-regulation of CASC19, NUCB1-AS1 and LINC01485 and down-regulation of VAV3-AS1, SPATA13-AS1, ZNF350-AS1 and LINC00677 (all p< 0.05).\nConclusion: Our findings map a vitiligo-related ceRNA landscape and pinpoints seven circulating lncRNAs with diagnostic promise. The results provide a foundation for probing non-coding RNA-mediated mechanisms and developing targeted therapies for vitiligo.", "claims": [{"id": "claim-method", "statement": "To identify differences between groups, the Student’s t-test was utilized, with a significance threshold set at p < 0.05.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40910032", "locator": "sections[13] \"Statistical Analysis\" paragraph 2", "quote": "To identify differences between groups, the Student’s t-test was utilized, with a significance threshold set at p < 0.05."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The miRNA expression profiling data were sourced from the NCBI GEO database, specifically dataset GSE141655, which includes blood samples from 10 vitiligo patients and 10 healthy individuals.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40910032", "locator": "sections[3] \"miRNA and mRNA/lncRNA Data Sets\" paragraph 2", "quote": "The miRNA expression profiling data were sourced from the NCBI GEO database, specifically dataset GSE141655, which includes blood samples from 10 vitiligo patients and 10 healthy individuals."}], "state": "complete"}, {"id": "claim-target-result", "statement": "A total of 454 DE-mRNAs (341 down-, 113 up-regulated), 22 DE-miRNAs (6 down-, 16 up-regulated), and 281 DE-lncRNAs (112 down-, 169 up-regulated) were identified.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:40910032", "locator": "sections[0] \"Abstract\" paragraph 4", "quote": "A total of 454 DE-mRNAs (341 down-, 113 up-regulated), 22 DE-miRNAs (6 down-, 16 up-regulated), and 281 DE-lncRNAs (112 down-, 169 up-regulated) were identified."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "A total of 454 DE-mRNAs (341 down-, 113 up-regulated), 22 DE-miRNAs (6 down-, 16 up-regulated), and 281 DE-lncRNAs (112 down-, 169 up-regulated) were identified.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:40910032"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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Candidate lncRNAs were validated by RT-qPCR in peripheral blood from 20 vitiligo patient", "lity. Candidate lncRNAs were validated by RT-qPCR in peripheral blood from 20 vitiligo patient", "raphPad Software, Inc., San Diego, CA, USA). RT-qPCR tests were conducted in triplicate or more.", "hPad Software, Inc., San Diego, CA, USA). RT-qPCR tests were conducted in triplicate or more."], "order_basis": "paper_text_order:1282", "id": "assay:1"}, {"role": "data_generation", "definition_id": "clinical_cohort", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["onships). Validation of lncRNA Expression in Blood Samples from Vitiligo Patients To assess the reliability", "n levels of the aforementioned 16 lncRNAs in blood samples from 20 vitiligo patients and from 20 healthy con", "els (Figure 5). 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