Temozolomide-Derived AIC Is Incorporated into Purine Synthesis in Glioblastoma.

{"sci_version": "1.0", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:40925600", "kind": "science-record", "revision": 1, "content_hash": "sha256:0ab83d3a21cf8417d10c72b750b153949f5b6e2ec3dbe638c506b4ad6c4e7147", "created_at": "2026-11-01T10:25:53Z", "updated_at": "2026-11-01T10:25:53Z", "generator": {"name": "corpus-assay-engine", "converter": "sep_to_sci_v1", "source_package_schema": "urn:bntrg:sep:1.0.0"}, "paper": {"state": "partial", "title": "Temozolomide-Derived AIC Is Incorporated into Purine Synthesis in Glioblastoma.", "doi": "10.1021/acs.chemrestox.5c00196", "pmid": "40925600", "authors": null, "journal": "Chemical research in toxicology", "year": 2025, "abstract": null, "claims": [{"id": "claim-1", "statement": "the headline numerical result the paper states for its main finding", "kind": "qualitative", "state": "blocked", "target": {"value": null, "unit": null, "tolerance": null, "source": null}, "evidence": []}], "gaps": ["abstract", 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["xml"], "capabilities": [], "data_gaps": ["source:pmid:40925600"]}, "gaps": ["corpus_record_absent"]}, "review": {"state": "partial", "machine_critique": {"status": "blocked", "providers": [{"provider": "minimax", "model": "MiniMax-M3.1-Flash", "prompt_version": "full-text-reading-v1", "prompt_hash": "c126541c4cf8b7670fc1c148143e265d834f65860c33786143319146377058a0", "source_hash": "c335de292035474b48e0c3c1ed46452c1eef07454e49e0ba3075e179e38607f1", "raw_response_sha256": "sha256:97394b88f3b61c3a4a91627c455da4609d8312fc65c74d0c9f81484e662aaeaf", "raw_response_chars": 5832}, {"provider": "minimax_alt", "model": "MiniMax-M3", "prompt_version": "full-text-reading-v1", "prompt_hash": "da224bc31574b8e7de19245aa9868572599f4ad7431365b3cca5ded5246f2919", "source_hash": "c335de292035474b48e0c3c1ed46452c1eef07454e49e0ba3075e179e38607f1", "raw_response_sha256": "sha256:31c1699c6e6a340a54cec6220b3c38b3f0d5bdd2b3db3ef6a6dc4ea09a3f3a18", "raw_response_chars": 76784}], "target_providers": [{"provider": "minimax", "model": "MiniMax-M3.1-Flash", "prompt_version": "replication-target-v2", "prompt_hash": "e6d6e6738a7652998042de0b67b7627d160f3e37b978729dabcdda6b9e71f1ea", "source_hash": "c335de292035474b48e0c3c1ed46452c1eef07454e49e0ba3075e179e38607f1", "raw_response": "{\"parent_value\":0,\"unit\":\"\",\"evidence\":\"\",\"target_result\":\"The paper states that AIC derived from temozolomide is incorporated into AMP and NAD+ in glioblastoma cell lines and that de novo purine synthesis is upregulated in GBM relative to normal brain, but it reports no numerical value for this main finding.\",\"number_stated\":false}", "reading": {"parent_value": 0, "unit": "", "evidence": "", "target_result": "The paper states that AIC derived from temozolomide is incorporated into AMP and NAD+ in glioblastoma cell lines and that de novo purine synthesis is upregulated in GBM relative to normal brain, but it reports no numerical value for this main finding.", "number_stated": false, "source_locator": null}, "recorded_at": "2026-10-31T15:34:58.744149+00:00"}, {"provider": "minimax_alt", "model": "MiniMax-M3", "prompt_version": "replication-target-v2", "prompt_hash": "3877f81be39daba5ac4a8030c8d36f7ef60719ec87e93528892e684899c2617e", "source_hash": "c335de292035474b48e0c3c1ed46452c1eef07454e49e0ba3075e179e38607f1", "raw_response": "<think>We need answer JSON only. Need parse task carefully. Need identify single numerical result headline paper states for main finding, registered target vague: \"the headline numerical result the paper states for its main finding\". Supplied source text appears abstract/results, perhaps no headline numerical result for main finding except perhaps numerical values in methods: 1142 samples, 105, 158, etc. Need determine intended extraction. User asks read supplied paper text and find single numerical result that replication of registered target has to reproduce. Registered target: headline numerical result paper states for its main finding. Need report headline number only, value paper itself states, unit printed. Exact contiguous quotation containing number. If no number for registered target, number_stated false, parent_value 0, unit/evidence empty, target_result what text states instead.\n\nWe need infer whether source contains a headline numerical result for main finding. Text title and abstract says TMZ-derived AIC incorporated into AMP/NAD+, upregulated pathways, no quantitative headline number. Numerical results include 24 h, sample counts, GraphPad 10, etc. Main finding perhaps \"AIC derived from TMZ is incorporated into AMP and NAD+ in glioblastoma cell lines\"; no number attached. Thus likely false. But wording perhaps benchmark designed to extract a specific number from article, likely numerical result might be hidden in source? Let's inspect full supplied text exactly. It includes abstract, methods snippets, results. No figures/results quantitative values except sample counts. Registered target says \"the headline numerical result the paper states for its main finding\" perhaps target result is an item like \"X% of AIC...\" but source text maybe no headline numeric result. We must not mistake sample counts for headline main finding. Need output target_result describing what text states instead, exact evidence empty per instruction if no number. JSON fields.\n\nCould potentially identify \"24 h after supplementation\" as numerical result? It's methodological time point, not headline result. \"all enzymes ... significantly upregulated\" no number. No p-value or fold-change. \"In total, 1142 ... 105 ... 158...\" not main finding headline. \"GraphPad PRSIM 10\" version. \"Figure S1\" etc. The prompt explicitly gives fallback if no number for registered target. Likely answer false.\n\nNeed formulate target_result: \"The text states that AIC derived from TMZ is incorporated into AMP and NAD+ in glioblastoma cell lines and that de novo and salvage purine synthesis pathways are active/upregulated in GBM; it does not state a numerical value for this main finding.\" Evidence must be empty because no number stated. unit empty, parent 0. JSON valid, no extra.\n\nBut ensure \"headline number only\" perhaps target result expected from paper's main finding maybe \"24 h\" is stated number linked to experiment. Yet prompt says registered target is \"the headline numerical result the paper states for its main finding\", perhaps benchmark asks identify numerical result perhaps in abstract omitted? Let's explore possibility article abstract's main quantitative finding not visible due truncation? Supplied source starts title and abstract, likely entire extraction only excerpt up through results. There may be headline number in figure not text. User intentionally says if no number, false. We should assess.\n\nCould \"the single numerical result\" refer to main finding likely \"AIC is incorporated into AMP and NAD+\" and perhaps numerical metric like 20% incorporation, but source does not give. Exact contiguous quote must include number and attach target. No such. Therefore false.\n\nNeed return only JSON object. target_result perhaps concise. Ensure schema number parent_value 0, booleans. Unit \"\", evidence \"\". Let's compose.</think>\n\n{\"parent_value\":0,\"unit\":\"\",\"evidence\":\"\",\"target_result\":\"The text states that TMZ-derived AIC is incorporated into AMP and NAD+ in glioblastoma cell lines and that purine synthesis pathways are upregulated in GBM, but it gives no numerical value for this main finding.\",\"number_stated\":false}", "reading": {"parent_value": 0, "unit": "", "evidence": "", "target_result": "The text states that TMZ-derived AIC is incorporated into AMP and NAD+ in glioblastoma cell lines and that purine synthesis pathways are upregulated in GBM, but it gives no numerical value for this main finding.", "number_stated": false, "source_locator": null}, "recorded_at": "2026-10-31T15:35:02.484586+00:00"}], "agreement": false, "disagreements": {"target_result": {"minimax": "No headline numerical result is stated for the main finding. The paper's main conclusion is that temozolomide-derived AIC is incorporated into AMP and NAD+ in glioblastoma cell lines and that de novo purine synthesis is upregulated in GBM relative to normal brain.", "minimax_alt": "The supplied text states the main finding qualitatively rather than numerically: AIC derived from TMZ is incorporated into AMP and NAD+ in glioblastoma cell lines, and de novo purine synthesis is upregulated in GBM relative to the normal brain. No numerical headline effect estimate is provided.", "decisive": false}, "physical_methods": {"minimax": ["Targeted mass spectrometry assay", "Isotope-enriched metabolite supplementation and tracer analysis", "Isotope-labeled temozolomide tracing"], "minimax_alt": ["Targeted mass spectrometry assay to quantify isotopic enrichment of AMP and NAD+", "Isotope-labeled TMZ tracing of AIC incorporation", "Cell-culture supplementation with isotope-enriched metabolites followed by tracking of label incorporation after 24 h"], "decisive": false}, "analysis_methods": {"minimax": ["RNA-sequencing gene-expression analysis", "UCSC Xena TCGA-GBM versus GTEx comparison", "Kaplan–Meier survival analysis", "Log-rank test", "GraphPad Prism 10"], "minimax_alt": ["Comparative analysis of log2-transformed “RSEM expected_count (DESeq2 standardized)” gene-expression data from TCGA-GBM and GTEx", "Kaplan–Meier survival analysis using median-split high- and low-expression cohorts", "Log-rank test"], "decisive": false}, "statistical_method": {"minimax": "Kaplan–Meier analysis with the log-rank test", "minimax_alt": "", "decisive": true}}, "agreement_basis": {"policy": "decisive_fields", "decisive_fields": ["work_type", "number_stated", "parent_value", "unit"]}, "blinded": true}, "human_critique": {"status": "not_run", "reviewer": null, "signed_at": null, "conflict_checked": false, "commentary": null}, "stages": [{"key": "target_materials", "status": "pending", "reason": null, "evidence": null}, {"key": "independent_readings", "status": "blocked", "reason": "The independent readings are incomplete or disagree; the source evidence must resolve the difference.", "evidence": {"provider_errors": []}}, {"key": "source_resolution", "status": "pending", "reason": null, "evidence": null}, {"key": "protocol_reconstruction", "status": "pending", "reason": null, "evidence": null}, {"key": "control_execution", "status": "pending", "reason": null, "evidence": null}, {"key": "control_comparison", "status": "pending", "reason": null, "evidence": null}, {"key": "publication", "status": "pending", "reason": null, "evidence": null}], "gaps": ["review.human_critique", "review.machine_critique.blocked"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "package:54d3ed2733ef88588ce173285d377383b52f22ac94762a683c714fe3c2eca6fd", "type": "sep_package", "label": "SEP v1 package for pmid:40925600"}], "activities": [{"id": "activity:conversion:pmid:40925600", "type": "conversion", "used": ["package:54d3ed2733ef88588ce173285d377383b52f22ac94762a683c714fe3c2eca6fd"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:minimax", "type": "model", "label": "minimax"}, {"id": "agent:minimax_alt", "type": "model", "label": "minimax_alt"}, {"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}], "source_run_id": "run-20261023T122449232913", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "3bd77af9-c2f2-4930-9108-5501013ae06b", "parent_hash": "54d3ed2733ef88588ce173285d377383b52f22ac94762a683c714fe3c2eca6fd", "fork_of": null, "generation": 1}, "gaps": ["abstract", "asset:source:pmid:40925600 not verified", "authors", "capability", "corpus_record_absent", "execution.not_run", "method", "review.human_critique", "review.machine_critique.blocked", "step:obtain_source_data blocked on data:not_downloaded", "step:run_analysis blocked on no owned computational capability is bound to this experiment", "step:run_analysis input 'materials:the source data behind the published result in pmid:40925600' does not resolve to a declared asset", "targets"]}