RHOJ enhances adhesion and proliferation capabilities and suppresses apoptosis of melanoma cells by activating the Rap1 signaling pathway.

{"sci_version": "1.0", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:40950707", "kind": "science-record", "revision": 1, "content_hash": "sha256:d544306e77c812aebf85eb4501ce91cbb8e084ae01891cd5aa6b32ffdd72d1aa", "created_at": "2026-11-01T10:25:53Z", "updated_at": "2026-11-01T10:25:53Z", "generator": {"name": "corpus-assay-engine", "converter": "sep_to_sci_v1", "source_package_schema": "urn:bntrg:sep:1.0.0"}, "paper": {"state": "partial", "title": "RHOJ enhances adhesion and proliferation capabilities and suppresses apoptosis of melanoma cells by activating the Rap1 signaling pathway.", "doi": "10.21037/tcr-2024-2692-b", "pmid": "40950707", "authors": null, "journal": "Translational cancer research", "year": 2025, "abstract": null, "claims": [{"id": "claim-1", "statement": "the headline numerical result the paper states for its main finding", "kind": "qualitative", "state": "blocked", "target": {"value": null, "unit": null, "tolerance": null, "source": 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{"dsl_operations": [], "converters": [], "readers": ["xml"], "capabilities": [], "data_gaps": ["source:pmid:40950707"]}, "gaps": ["corpus_record_absent"]}, "review": {"state": "partial", "machine_critique": {"status": "blocked", "providers": [{"provider": "minimax", "model": "MiniMax-M3.1-Flash", "prompt_version": "full-text-reading-v1", "prompt_hash": "90ebe2aad943882aad61c558b286b30e4f71deee5ff2b4b67b9a47c4ce827ac6", "source_hash": "c98d41714a87227a231e1242ef67f2a39159ea855627778fcd9285c717b1c583", "raw_response_sha256": "sha256:a0163fee20efbac246a717eabe4426feebd61c1245fdeb414e6bb0da0783f846", "raw_response_chars": 7491}, {"provider": "minimax_alt", "model": "MiniMax-M3", "prompt_version": "full-text-reading-v1", "prompt_hash": "647c1fb43c94da046a8adc4db1be86546c21c8aa122da52c0eddccc8ac828634", "source_hash": "c98d41714a87227a231e1242ef67f2a39159ea855627778fcd9285c717b1c583", "raw_response_sha256": "sha256:c4efe952fe108f454360754d334975dcf2a6a9b3cf3bad10d3f6a9fbc0b79cb6", "raw_response_chars": 48236}], "target_providers": [{"provider": "minimax", "model": "MiniMax-M3.1-Flash", "prompt_version": "replication-target-v2", "prompt_hash": "3b85b596c05d0ad33b7ab58d0374a6cf8149145468dfdf55b77adc6928a87d56", "source_hash": "c98d41714a87227a231e1242ef67f2a39159ea855627778fcd9285c717b1c583", "raw_response": "{\"parent_value\":0,\"unit\":\"\",\"evidence\":\"\",\"target_result\":\"The paper states that RHOJ promotes melanoma cell adhesion and proliferation while inhibiting apoptosis through activation of the Rap1 signaling pathway, but it does not provide a numerical result for this main finding.\",\"number_stated\":false}", "reading": {"parent_value": 0, "unit": "", "evidence": "", "target_result": "The paper states that RHOJ promotes melanoma cell adhesion and proliferation while inhibiting apoptosis through activation of the Rap1 signaling pathway, but it does not provide a numerical result for this main finding.", "number_stated": false, "source_locator": null}, "recorded_at": "2026-10-31T15:35:34.851514+00:00"}, {"provider": "minimax_alt", "model": "MiniMax-M3", "prompt_version": "replication-target-v2", "prompt_hash": "36811481c09615e942b06dcff9c40fbbc3d3028a548e03ecf5240e886666caa0", "source_hash": "c98d41714a87227a231e1242ef67f2a39159ea855627778fcd9285c717b1c583", "raw_response": "<think>We need answer JSON only. Need identify single numerical result headline for main finding. Source is odd, abstract lacks numerical headline. It has sample sizes 461 melanoma, 558 normal melanocyte. Registered target headline numerical result main finding. Need infer perhaps paper has numerical result in supplied text? No explicit effect sizes, p values, fold changes. It asks value paper itself states for registered target. The numerical result likely sample count? But target is \"headline numerical result the paper states for its main finding\" maybe task expects extract one number tied to headline finding. Source text includes RHOJ expression and sample analysis but no quantified RHOJ result. Thus number_stated false, parent_value 0, unit/evidence empty, target_result says text states qualitative findings and no numeric result. Need exact JSON, no invented source. Could there be one numerical result: 461 melanoma tissue samples and 558 samples normal melanocyte. But that's methods cohort, not headline numerical result. The abstract/conclusion doesn't state numerical effect. We need carefully parse instruction: \"Read supplied paper text and find the single numerical result that a replication of the registered target has to reproduce. Report the headline number only: the value the paper itself states for the registered target, not a value you calculated, converted, averaged or reconstructed.\" Registered target = headline numerical result paper states for its main finding. We should identify if headline numerical result is maybe \"RHOJ significantly upregulated\" but no number. Therefore false.\n\nNeed target_result likely \"The text states that RHOJ is upregulated in melanoma cells and that knockdown reduces viability, proliferation, and adhesion and increases apoptosis, but it reports no numerical value for this main finding.\" Unit empty, evidence empty, parent 0. Ensure JSON only.</think>\n\n{\"parent_value\":0,\"unit\":\"\",\"evidence\":\"\",\"target_result\":\"The text states that RHOJ is significantly upregulated in melanoma cells and that RHOJ knockdown reduces cell viability, proliferation, and adhesion while increasing apoptosis, but it gives no numerical value for this main finding.\",\"number_stated\":false}", "reading": {"parent_value": 0, "unit": "", "evidence": "", "target_result": "The text states that RHOJ is significantly upregulated in melanoma cells and that RHOJ knockdown reduces cell viability, proliferation, and adhesion while increasing apoptosis, but it gives no numerical value for this main finding.", "number_stated": false, "source_locator": null}, "recorded_at": "2026-10-31T15:35:37.560188+00:00"}], "agreement": false, "disagreements": {"target_result": {"minimax": "RHOJ promotes melanoma cell adhesion and proliferation while inhibiting apoptosis through the activation of the Rap1 signaling pathway", "minimax_alt": "No headline numerical result is stated in the supplied text; the headline finding is that RHOJ promotes melanoma cell adhesion and proliferation while inhibiting apoptosis through the activation of the Rap1 signaling pathway.", "decisive": false}, "physical_methods": {"minimax": ["A375 cells were transfected with si-RHOJ, with or without the addition of a Rap1 signaling pathway activator.", "Cell viability was assessed using the Cell Counting Kit-8 (CCK-8) assay, while cell proliferation was measured using the 5-ethynyl-2'-deoxyuridine (EdU) assay.", "Apoptosis was evaluated by flow cytometry, and cell adhesion was determined using a cell adhesion detection kit.", "The expression of relevant genes was analyzed by real-time polymerase chain reaction (PCR), Western blot, and immunofluorescence techniques."], "minimax_alt": ["A375 melanoma cell in vitro model", "si-RHOJ transfection with or without a Rap1 signaling pathway activator", "Cell Counting Kit-8 (CCK-8) assay for cell viability", "5-ethynyl-2'-deoxyuridine (EdU) assay for cell proliferation", "Flow cytometry for apoptosis and cell-cycle analysis", "Cell adhesion detection kit", "Real-time polymerase chain reaction (PCR)", "Western blot", "Immunofluorescence"], "decisive": false}, "analysis_methods": {"minimax": ["Gene Expression Omnibus (GEO) dataset analysis", "Gene Expression Profiling Interactive Analysis (GEPIA) database analysis", "STRING database analysis", "Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis", "independent Student's t-test", "single-factor analysis of variance (ANOVA) followed by a Least Significant Difference (LSD) test"], "minimax_alt": ["GEO and GEPIA differential-expression analysis", "STRING database analysis for RHOJ-associated genes", "Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis", "ImageJ densitometric quantification of Western blot protein levels"], "decisive": false}, "statistical_method": {"minimax": "Independent Student's t-test for two groups; single-factor analysis of variance (ANOVA) followed by a Least Significant Difference (LSD) test for comparisons across three or more groups", "minimax_alt": "Prism was used for statistical analysis. An independent Student's t-test was used for two-group comparisons, and single-factor analysis of variance (ANOVA) followed by a Least Significant Difference (LSD) test was used for comparisons of three or more groups, with P < 0.05 considered significant.", "decisive": true}}, "agreement_basis": {"policy": "decisive_fields", "decisive_fields": ["work_type", "number_stated", "parent_value", "unit"]}, "blinded": true}, "human_critique": {"status": "not_run", "reviewer": null, "signed_at": null, "conflict_checked": false, "commentary": null}, "stages": [{"key": "target_materials", "status": "pending", "reason": null, "evidence": null}, {"key": "independent_readings", "status": "blocked", "reason": "The independent readings are incomplete or disagree; the source evidence must resolve the difference.", "evidence": {"provider_errors": []}}, {"key": "source_resolution", "status": "pending", "reason": null, "evidence": null}, {"key": "protocol_reconstruction", "status": "pending", "reason": null, "evidence": null}, {"key": "control_execution", "status": "pending", "reason": null, "evidence": null}, {"key": "control_comparison", "status": "pending", "reason": null, "evidence": null}, {"key": "publication", "status": "pending", "reason": null, "evidence": null}], "gaps": ["review.human_critique", "review.machine_critique.blocked"]}, "execution": {"state": "absent", "status": "not_run", "mode": null, "runs": [], "gaps": ["execution.not_run"]}, "provenance": {"entities": [{"id": "package:bb815f3e058c225f79aff22f4275f7e37a37aeb511eec20baffeb067699415d4", "type": "sep_package", "label": "SEP v1 package for pmid:40950707"}], "activities": [{"id": "activity:conversion:pmid:40950707", "type": "conversion", "used": ["package:bb815f3e058c225f79aff22f4275f7e37a37aeb511eec20baffeb067699415d4"], "agent": "agent:corpus-assay-engine"}], "agents": [{"id": "agent:minimax", "type": "model", "label": "minimax"}, {"id": "agent:minimax_alt", "type": "model", "label": "minimax_alt"}, {"id": "agent:corpus-assay-engine", "type": "software", "label": "Corpus assay engine"}], "source_run_id": "run-20261023T122449232913", "dsl_catalogue_fingerprint": "343fb30756ba6ec3", "capability_table_fingerprint": "2cb3e42f047d2ecf", "snapshot_id": "ba34b49490d9bfaf"}, "lineage": {"parent_id": "17d0c560-b5b2-4c47-bfa2-f829d7e6fb8b", "parent_hash": "bb815f3e058c225f79aff22f4275f7e37a37aeb511eec20baffeb067699415d4", "fork_of": null, "generation": 1}, "gaps": ["abstract", "asset:source:pmid:40950707 not verified", "authors", "capability", "corpus_record_absent", "execution.not_run", "method", "review.human_critique", "review.machine_critique.blocked", "step:obtain_source_data blocked on data:not_downloaded", "step:run_analysis blocked on no owned computational capability is bound to this experiment", "step:run_analysis input 'materials:the source data behind the published result in pmid:40950707' does not resolve to a declared asset", "targets"]}