SSBP1 is a prognostic biomarker and promotes the progression of lung adenocarcinoma via p53 signaling pathway.

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test\",\"Benjamini-Hochberg false-discovery-rate correction\",\"Kaplan-Meier survival analysis\",\"Log-rank test\",\"Univariate Cox proportional hazards regression\",\"Multivariate Cox proportional hazards regression\",\"Receiver operating characteristic curve analysis\",\"Area under the ROC curve\",\"KEGG pathway enrichment analysis\",\"Hierarchical clustering\",\"Single-cell RNA-sequencing analysis\",\"Student's t-test\",\"Two-way ANOVA\"],\"statistical_method\":\"Non-parametric Wilcoxon rank-sum tests, with signed-rank tests for paired data when useful, were used to test SSBP1 differential expression between tumor and normal tissues; the Benjamini-Hochberg approach was used to control the false discovery rate.\",\"method_evidence\":\"To assess the SSBP1 differential expression between tumor and normal tissues in different types of cancer, we used non-parametric statistical tests. These included Wilcoxon rank-sum tests to compare the levels of expression in tumor and normal tissues, and signed-rank tests to take paired data into consideration when useful. The Benjamini-Hochberg approach was used to control the false discovery rate (FDR) and further adjust statistical significance for multiple comparisons.\",\"repositories\":[{\"repository\":\"UCSC Xena database\",\"accession\":\"https://xenabrowser.net/datapages/\",\"data_description\":\"Normalized RNA-sequencing results from multiple cancer types in TCGA, together with SSBP1 expression data and clinical variables including OS, DSS, tumor stage, age, gender, treatment history, and tumor type.\",\"evidence\":\"The data for the pan-cancer expression profiles came from the UCSC Xena database, which contains normalized RNA sequencing (RNA-Seq) results from many different cancer types found in The Cancer Genome Atlas (TCGA) (https://xenabrowser.net/datapages/). For each kind of tumor that was accessible, we extracted the SSBP1 expression data and associated clinical variables, including OS, DSS, tumor stage, age, gender, treatment history, and tumor type.\",\"publicly_downloadable\":false},{\"repository\":\"The Cancer Genome Atlas\",\"accession\":\"unknown\",\"data_description\":\"Tumor and normal-tissue expression data used to compare SSBP1 expression in LUAD and other cancers; the pan-cancer analysis also used associated survival and clinical variables.\",\"evidence\":\"The data for the pan-cancer expression profiles came from the UCSC Xena database, which contains normalized RNA sequencing (RNA-Seq) results from many different cancer types found in The Cancer Genome Atlas (TCGA) (https://xenabrowser.net/datapages/).\",\"publicly_downloadable\":false},{\"repository\":\"GTEx\",\"accession\":\"unknown\",\"data_description\":\"Dataset used with TCGA and the GSE datasets to compare SSBP1 expression in LUAD tumor tissues and normal lung tissues; its exact accession and detailed contents are not stated.\",\"evidence\":\"In this study, comprehensive analysis using multiple datasets, including TCGA, GTEx, GSE19188, and GSE63459, consistently demonstrated that SSBP1 expression is elevated in LUAD, distinguishing tumor tissues from normal lung tissues.\",\"publicly_downloadable\":false},{\"repository\":\"GSE19188\",\"accession\":\"GSE19188\",\"data_description\":\"Dataset used in the LUAD expression analysis alongside TCGA, GTEx, and GSE63459; the source does not specify its detailed contents or an explicit download statement.\",\"evidence\":\"In this study, comprehensive analysis using multiple datasets, including TCGA, GTEx, GSE19188, and GSE63459, consistently demonstrated that SSBP1 expression is elevated in LUAD, distinguishing tumor tissues from normal lung tissues.\",\"publicly_downloadable\":false},{\"repository\":\"GSE63459\",\"accession\":\"GSE63459\",\"data_description\":\"Dataset used in the LUAD expression analysis alongside TCGA, GTEx, and GSE19188; the source does not specify its detailed contents or an explicit download statement.\",\"evidence\":\"In this study, comprehensive analysis using multiple datasets, including TCGA, GTEx, GSE19188, and GSE63459, consistently demonstrated that SSBP1 expression is elevated in LUAD, distinguishing tumor tissues from normal lung tissues.\",\"publicly_downloadable\":false}],\"availability_statement\":\"The datasets generated during and/or analyzed during the current study are available from the corresponding author upon reasonable request.\",\"data_on_request\":true,\"source_claims\":[\"SSBP1, a mitochondrial single-strand binding protein, was found to be significantly overexpressed in lung adenocarcinoma (LUAD) tissues, indicating its potential as a diagnostic biomarker.\",\"In this study, comprehensive analysis using multiple datasets, including TCGA, GTEx, GSE19188, and GSE63459, consistently demonstrated that SSBP1 expression is elevated in LUAD, distinguishing tumor tissues from normal lung tissues.\",\"Moreover, high SSBP1 expression was strongly associated with worse overall survival (OS), disease-specific survival (DSS), and progression-free interval (PFI), further supporting its role as a prognostic marker.\",\"Multivariate Cox regression analysis confirmed that SSBP1 remains an independent predictor of poor prognosis in LUAD patients.\",\"Functional studies showed that knockdown of SSBP1 in LUAD cell lines (A549 and H1299) led to significantly reduced proliferation and migration, coupled with downregulation of key cell cycle regulators such as CDK4 and CDK6.\",\"Additionally, SSBP1 silencing inhibited LUAD cell metastasis through the p53 signaling pathway, affecting the expression of EMT markers like N-cadherin and E-cadherin.\",\"The datasets generated during and/or analyzed during the current study are available from the corresponding author upon reasonable request.\"],\"missing_information\":[\"The supplied source does not state a numerical effect size, confidence interval, hazard ratio, or p-value for the headline LUAD overexpression result.\",\"No accession is printed for TCGA, GTEx, or UCSC Xena beyond the printed Xena URL.\",\"The text does not explicitly state that the UCSC Xena, TCGA, GTEx, GSE19188, or GSE63459 data can be downloaded without an application, embargo, or request.\",\"The individual data contents of GTEx, GSE19188, and GSE63459 are not separately specified.\",\"The paper's prognostic-analysis section discusses hepatocellular carcinoma rather than explicitly stating which survival method was applied specifically to the LUAD headline result.\"],\"evidence_spans\":[\"SSBP1, a mitochondrial single-strand binding protein, was found to be significantly overexpressed in lung adenocarcinoma (LUAD) tissues, indicating its potential as a diagnostic biomarker.\",\"In this study, comprehensive analysis using multiple datasets, including TCGA, GTEx, GSE19188, and GSE63459, consistently demonstrated that SSBP1 expression is elevated in LUAD, distinguishing tumor tissues from normal lung tissues.\",\"Moreover, high SSBP1 expression was strongly associated with worse overall survival (OS), disease-specific survival (DSS), and progression-free interval (PFI), further supporting its role as a prognostic marker.\",\"Multivariate Cox regression analysis confirmed that SSBP1 remains an independent predictor of poor prognosis in LUAD patients.\",\"To assess the SSBP1 differential expression between tumor and normal tissues in different types of cancer, we used non-parametric statistical tests. 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