Immunoproteomic Identification of Scedosporium boydii Antigens with Potential Diagnostic Interest in Cystic Fibrosis Patients.

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Current serodiagnostic methods, such as counterimmunoelectrophoresis and ELISA using crude extracts, lack standardization and commercial availability and often show cross-reactivity with other fungi. This study aimed to identify specific Scedosporium boydii antigens with potential for serodiagnosis in pwCF. An immunoproteomic approach combining two-dimensional polyacrylamide gel electrophoresis, Western blot, and mass spectrometry was used to analyze the antigenic profile of S. boydii against sera from pwCF with positive cultures for Scedosporium/Lomentospora (Scedo+) and mice intravenously infected with these fungi. We identified 22 proteins specifically recognized by sera from pwCF Scedo+ and mice infected with S. boydii, but not by sera from pwCF or mice infected with Aspergillus spp. or uninfected controls. These proteins are primarily involved in metabolism and exhibit catalytic activity. The most prevalent antigens were the heat shock protein 70, 6-phosphogluconate dehydrogenase, 3-ketoacyl-CoA thiolase, and phosphoenolpyruvate carboxykinase, recognized by 67-81% of pwCF Scedo + sera with minimal cross-reactivity to negative samples. This work identifies promising candidates for the specific serodiagnosis of Scedosporium/Lomentospora infections in pwCF. Data are available via ProteomeXchange (PXD053392).", "claims": [{"id": "claim-data-access", "statement": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD053392.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:40997942", "locator": "sections[29] \"Acknowledgments\" paragraph 2", "quote": "The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD053392."}], "state": "complete"}, {"id": "claim-target-result", "statement": "The study identified 22 S. boydii proteins specifically recognized by sera from pwCF with Scedosporium/Lomentospora-positive cultures and mice infected with S. boydii.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:40997942", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "We identified 22 proteins specifically recognized by sera from pwCF Scedo+ and mice infected with S. boydii, but not by sera from pwCF or mice infected with Aspergillus spp. or uninfected controls."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "The study identified 22 S. boydii proteins specifically recognized by sera from pwCF with Scedosporium/Lomentospora-positive cultures and mice infected with S. boydii.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:40997942"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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