Exometabolome and Molecular Signatures Associated with HPV 16 in Cervical Cancer: Integrative Metabolomic and Transcriptomic Analysis for Biomarker Discovery.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:41097329", "kind": "science-record", "revision": 4, "content_hash": "sha256:95756751cacf52f3e9104af20aa956a86e8a47bee78c0668fa27985f7452c088", "created_at": "2026-11-01T20:31:09Z", "updated_at": "2026-11-01T22:57:19Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Exometabolome and Molecular Signatures Associated with HPV 16 in Cervical Cancer: Integrative Metabolomic and Transcriptomic Analysis for Biomarker Discovery.", "doi": "10.3390/molecules30193909", "pmid": "41097329", "authors": ["Adán Arizmendi-Izazaga", "Napoleón Navarro-Tito", "Gabriela Elizabeth Campos-Viguri", "Hilda Jiménez-Wences", "Macdiel Emilio Acevedo-Quiroz", "Eric Genaro Salmerón-Bárcenas", "Berenice Illades-Aguiar", "Marco Antonio Leyva-Vázquez", "Julio Ortiz-Ortiz"], "journal": "Molecules (Basel, Switzerland)", "year": 2025, "abstract": "Cervical cancer (CC) represents a major public health concern, ranking as the fourth most frequently diagnosed cancer and one of the leading causes of cancer-related mortality among middle-aged women worldwide. CC is caused by persistent infection with high-risk human papillomaviruses (HR-HPVs), with HPV 16 being the cause of more than 50% of CC cases. In this study, the exometabolome of the HPV 16-positive cell lines SiHa and Ca Ski, as well as the HPV 16-negative control cell line C-33 A, was evaluated. The exometabolome was validated through molecular signatures using a transcriptomic approach to identify genes encoding cellular metabolic enzymes. The exometabolome was analyzed using 1H nuclear magnetic resonance spectroscopy (1H-NMR). Exometabolomic profiles were subsequently compared through both multivariate and univariate statistical analyses to identify significant differences between cell lines. Molecular signatures were analyzed from the GSE9750 dataset obtained from the GEO database. Exometabolic profiling of the HPV 16 positive cell lines showed higher concentrations of leucine, isoleucine, valine, lysine, methionine, glutamine, ornithine, choline, glucose, and tryptophan. An expression analysis showed increased expression of enzymes involved in amino acid synthesis, the tricarboxylic acid cycle, glycolysis, the pentose phosphate pathway, galactose metabolism, and HIF-1α. These data suggest metabolites and metabolism-associated genes that can be used as non-invasive, stable diagnostic and prognostic biomarkers, as well as therapeutic targets for CC in the presence of HPV 16.", "claims": [{"id": "claim-method", "statement": "PCA was used to examine intrinsic variability within the dataset, to observe clustering or separation trends, and to identify outliers. OPLS-DA was applied to minimize the potential contribution of intergroup variability and to improve the separation between sample groups further.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:41097329", "locator": "sections[12] \"4.4. 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OPLS-DA was applied to minimize the potential contribution of intergroup variability and to improve the separation between sample groups further."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The links to the expression data were posted in the Section 4.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:41097329", "locator": "sections[23] \"Data Availability Statement\" paragraph 2", "quote": "The links to the expression data were posted in the Section 4."}], "state": "complete"}, {"id": "claim-target-result", "statement": "Exometabolic profiling of the HPV 16 positive cell lines showed higher concentrations of leucine, isoleucine, valine, lysine, methionine, glutamine, ornithine, choline, glucose, and tryptophan.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:41097329", "locator": "sections[0] \"Abstract\" paragraph 1", "quote": "Exometabolic profiling of the HPV 16 positive cell lines showed higher concentrations of leucine, isoleucine, valine, lysine, methionine, glutamine, ornithine, choline, glucose, and tryptophan."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": "Exometabolic profiling of the HPV 16 positive cell lines showed higher concentrations of leucine, isoleucine, valine, lysine, methionine, glutamine, ornithine, choline, glucose, and tryptophan.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:41097329"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. 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