Single-cell RNA-seq reveals the piperlongumine is a potential drug for ischemic stroke.

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The aim of this study was to explore the underlying molecular mechanisms and potentially effective therapeutic drugs for ischemic stroke. Single-cell seq data (GSE174574) were downloaded from the Gene Expression Omnibus (GEO) database, and dimensionality reduction clustering was performed after quality control. Eighteen cell clusters were identified, which were annotated into 9 cell types according to specific marker genes. In addition, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Set Enrichment Analysis (GSEA) enrichment analysis showed that apoptosis was significantly increased after ischemic brain injury, while p53 signaling pathway, TNF-a signaling pathway and mitogen-activated protein kinase (MAPK) signaling pathway may play an important role in ischemic stroke. Furthermore, Connectivity Map (CMap) analysis and molecular docking suggested that piperlongumine might be an effective drug for the treatment of ischemic stroke by binding to the proteins encoded by Actb and Cflar. Finally, in vitro and in vivo experiments conformed the effectiveness of piperlongumine. This study provided new ideas for the treatment of ischemic stroke.", "claims": [{"id": "claim-method", "statement": "Statistical analyses were performed using one-way ANOVA followed by Tukey’s post hoc test for multiple comparisons or Student’s t-test for pairwise comparisons, as appropriate, utilizing R software (version 4.3.0).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:41575950", "locator": "sections[16] \"2.14. 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Finally, in vitro and in vivo experiments conformed the effectiveness of p", ". In accordance with national standards, the mice were fed commercial laboratory animal feed that w", "d. For all surgeries, anesthesia was induced in mice using 3% isoflurane and maintained with 2% i", "was sterilized by cobalt 60 irradiation. The mouse drinking water was pH 2.5–3.0 acidified. 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