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pmid:41585413

MoGAAAP: a modular Snakemake workflow for automated genome assembly and annotation with quality assessment.

Dirk-Jan M van Workum · Kuntal K Dey · Alexander Kozik · Dean O Lavelle · Dick de Ridder · M Eric Schranz · Richard W Michelmore · Sandra Smit

NAR genomics and bioinformatics · 2026 doi:10.1093/nargab/lqag008

Paper

complete

Experiment

partial

Data

partial

Analysis

absent

Execution

absent

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Record details sci_version · id · kind · revision · content_hash · schema · created_at · updated_at · generator
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science-record
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title
MoGAAAP: a modular Snakemake workflow for automated genome assembly and annotation with quality assessment.
doi
10.1093/nargab/lqag008
pmid
41585413
authors array · 8 items
[0]
Dirk-Jan M van Workum
[1]
Kuntal K Dey
[2]
Alexander Kozik
[3]
Dean O Lavelle
[4]
Dick de Ridder
[5]
M Eric Schranz
[6]
Richard W Michelmore
[7]
Sandra Smit
journal
NAR genomics and bioinformatics
year
2026
abstract
With the current speed of sequencing, there is a desire for standardized and automated genome assembly and annotation to produce high-quality genomes as input for comparative (pan)genomics. Therefore, we created a convenience pipeline using existing tools that creates annotated genome assemblies from HiFi (and optionally ultra-long ONT and/or Hi-C) reads for a set of related individuals as well as a related reference genome. Our pipeline is species-agnostic and generates an extensive quality assessment report that can be used for manual filtering and refinement of the assembly and annotation. It includes statistics for individual completeness and contamination assessments as well as a concise pangenome view. The pipeline is implemented in Snakemake and available with a GPLv3 licence at GitHub under github.com/dirkjanvw/MoGAAAP, at Zenodo under doi.org/10.5281/zenodo.14833021, and can be installed through Bioconda.
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The novel L. serriola genome, which is described as a use case in the Supplementary data, has been published at NCBI GenBank (GCA_051521515.1). Underlying HiFi and ONT sequencing data has been published under BioProject PRJNA412928. HTML reports created by MoGAAAP for the use cases described in the Supplementary data are available through data. 4TU (DOI: 10.4121/4b39da65-2eef-4e05-8583-25f8850cf932).
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The novel L. serriola genome, which is described as a use case in the Supplementary data, has been published at NCBI GenBank (GCA_051521515.1). Underlying HiFi and ONT sequencing data has been published under BioProject PRJNA412928. HTML reports created by MoGAAAP for the use cases described in the Supplementary data are available through data. 4TU (DOI: 10.4121/4b39da65-2eef-4e05-8583-25f8850cf932).
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The novel L. serriola genome, which is described as a use case in the Supplementary data, has been published at NCBI GenBank (GCA_051521515.1). Underlying HiFi and ONT sequencing data has been published under BioProject PRJNA412928. HTML reports created by MoGAAAP for the use cases described in the Supplementary data are available through data. 4TU (DOI: 10.4121/4b39da65-2eef-4e05-8583-25f8850cf932).
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The novel L. serriola genome, which is described as a use case in the Supplementary data, has been published at NCBI GenBank (GCA_051521515.1). Underlying HiFi and ONT sequencing data has been published under BioProject PRJNA412928. HTML reports created by MoGAAAP for the use cases described in the Supplementary data are available through data. 4TU (DOI: 10.4121/4b39da65-2eef-4e05-8583-25f8850cf932).
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