Unveiling the prognostic role of FABP4 in early-onset colorectal cancer through big data analysis and preliminary clinical validation.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:41607549", "kind": "science-record", "revision": 3, "content_hash": "sha256:dc4f7292f7d1cdc81b23815fdba997f552590e7476bb9bdb1c40c2d468214eea", "created_at": "2026-11-01T20:31:34Z", "updated_at": "2026-11-01T22:15:23Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "Unveiling the prognostic role of FABP4 in early-onset colorectal cancer through big data analysis and preliminary clinical validation.", "doi": "10.3389/fonc.2025.1689952", "pmid": "41607549", "authors": ["Yu Wu", "Weiwei Zou", "Shengjun Zhang", "Lipeng Zhao", "Shaohua He", "Fan Yao", "Peilin Qing", "Yixin Li", "Jie Li", "Xiao-Liang Xing"], "journal": "Frontiers in oncology", "year": 2025, "abstract": "Background: Early-onset colorectal cancer (EOCRC), characterized by greater aggressiveness and advanced stage at diagnosis, is increasing globally. This study aimed to identify suitable prognostic biomarkers for EOCRC.\nMethods: Gene expression and clinical data from TCGA and GEO (GSE39582, GSE17536, and GSE17537) datasets were analyzed. Differential expression, univariate and multivariate Cox regression, and correlation analyses were performed. Immune status was evaluated using ESTIMATE and CONSENSUS TME algorithm. Immunotherapy and chemotherapy response was predicted via the TIDE and oncoPredict algorithm, respectively. Candidate signatures were validated in clinical samples from three EOCRC patients using qRT-PCR.\nResults: Fatty Acid Binding Protein 4 (FABP4) was identified as an independent prognostic factor for poor overall survival in EOCRC patients. A prognostic model based on FABP4 demonstrated good predictive accuracy for 1-, 3-, and 5-year survival in both training and validation sets. The high-FABP4 expression was negative correlated TIDE scores, suggesting EOCRC patients with high expression of FABP4 maybe benefit from immunotherapy. Furthermore, 66 chemotherapeutic agents showed significant negative correlations with FABP4 expression. Validation in patient samples confirmed the coordinated upregulation of FABP4 and its associated genes ADIPOQ and IGF1.\nConclusion: FABP4 is a promising independent prognostic biomarker for EOCRC, associated with an immunosuppressive microenvironment and maybe a potential guidance for immunotherapy and chemotherapy selection. Further multi-center prospective studies are warranted to validate its clinical utility.", "claims": [{"id": "claim-method", "statement": "Univariate and multivariate Cox regression analyses were conducted to identify signatures independently associated with overall survival (OS).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:41607549", "locator": "sections[4] \"Cox regression analysis\" paragraph 2", "quote": "Univariate and multivariate Cox regression analyses were conducted to identify signatures independently associated with overall survival (OS)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The original contributions presented in the study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding authors.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:41607549", "locator": "sections[21] \"Data availability statement\" paragraph 2", "quote": "The original contributions presented in the study are included in the article/Supplementary Material. Further inquiries can be directed to the corresponding authors."}], "state": "complete"}, {"id": "claim-target-result", "statement": "FABP4 was identified as an independent prognostic factor for poor overall survival in EOCRC patients.", "kind": "qualitative", "target": null, "evidence": [{"asset": "source:pmid:41607549", "locator": "sections[0] \"Abstract\" paragraph 3", "quote": "Fatty Acid Binding Protein 4 (FABP4) was identified as an independent prognostic factor for poor overall survival in EOCRC patients."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "complete", "objective": "FABP4 was identified as an independent prognostic factor for poor overall survival in EOCRC patients.", "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:41607549"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "complete", "blocked_on": null, "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE17536"], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": ["data:geo:GSE17536"], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": []}, "data": {"state": "partial", "assets": [{"id": "source:pmid:41607549", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.3389/fonc.2025.1689952", "sha256": "sha256:7d43a28a0116dc5a17230d9c7becb11ff67a8ecbdd4c3e3cc965b7b818b76d37", "bytes": 46434, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.3389/fonc.2025.1689952", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC12836056", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "data:geo:GSE17536", "role": "raw", "format": "geo-series", "media_type": "application/vnd.ncbi.geo-series+json", "uri": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE17536", "sha256": null, "bytes": null, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "GSE17536", "version": null, "note": "The original contributions presented in the study are included in the article/Supplementary Material. 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