MUDENG Expression Profiling in Cohorts and Brain Tumor Biospecimens to Evaluate Its Role in Cancer.

{"sci_version": "1.2", "schema": "https://corpus.bntrg.co.uk/sci/1.0/sci.schema.json", "id": "pmid:31616474", "kind": "science-record", "revision": 7, "content_hash": "sha256:4a9d7bfdaa9c1acc7d653309d945c9bbc47414f27d8a1d6f2c0eeb80a6696906", "created_at": "2026-11-01T15:40:50Z", "updated_at": "2026-11-01T22:00:54Z", "generator": {"name": "corpus-assay-engine", "run_id": "reading-sci-2026-11-01"}, "paper": {"state": "complete", "title": "MUDENG Expression Profiling in Cohorts and Brain Tumor Biospecimens to Evaluate Its Role in Cancer.", "doi": "10.3389/fgene.2019.00884", "pmid": "31616474", "authors": ["Juhyun Shin", "Jun-Ha Choi", "Seunghwa Jung", "Somi Jeong", "Jeongheon Oh", "Do-Young Yoon", "Man Hee Rhee", "Jaehong Ahn", "Se-Hyuk Kim", "Jae-Wook Oh"], "journal": "Frontiers in genetics", "year": 2019, "abstract": "Mu-2-related death-inducing gene (MUDENG, MuD) has been reported to be involved in the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-associated apoptotic pathway of glioblastoma multiforme (GBM) cells; however, its expression level, interactors, and role in tumors are yet to be discovered. To investigate whether MuD expression correlates with cancer progression, we analyzed The Cancer Genome Atlas (TCGA) database using UALCAN and Gene Expression Profiling Interactive Analysis (GEPIA). Differential expression of MuD was detected in 6 and 10 cancer types, respectively. Validation performed using data from the Gene Expression Omnibus database showed that MuD expression is downregulated in KIRC tumor and correlate with higher chance of survival. Upregulation of MuD expression in GBM tumors was detected through GEPIA and high MuD expression correlated with higher survival in proneural GBM, whereas the opposite was observed in classical GBM subtype. GBM biospecimens analysis shows that MuD protein level was upregulated in three of six specimens, whereas mRNA level remained relatively unaltered. Therefore, MuD may exert differential effects according to subtypes, and/or be subjected to post-translational regulation in GBM. Correlation analysis between GBM cohort database and experiments using GBM cell lines revealed its positive effect on regulation of protein phosphatase 2 regulatory subunit B'Epsilon (PPP2R5E) and son of sevenless homolog 2 (SOS2). STRING database analysis indicated that the components of adaptor protein complexes putatively interacted with MuD but showed no correlation in terms of survival of patients with different GBM subtypes. In summary, we analyzed the expression of MuD in publicly available cancer patient data sets, GBM cell lines, and biospecimens to demonstrate its potential role as a biomarker for cancer prognosis and identified its candidate interacting molecules.", "claims": [{"id": "claim-method", "statement": "Cox proportional hazard analysis was performed with GBM-BioDP provided at the Glioblastoma Bio Discovery Portal (https://gbm-biodp.nci.nih.gov/) (Celiku et al., 2014).", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31616474", "locator": "sections[4] \"Statistical Analysis\" paragraph 2", "quote": "Cox proportional hazard analysis was performed with GBM-BioDP provided at the Glioblastoma Bio Discovery Portal (https://gbm-biodp.nci.nih.gov/) (Celiku et al., 2014)."}], "state": "complete"}, {"id": "claim-data-access", "statement": "The datasets generated for this study are available on request to the corresponding author.", "kind": "methodological", "target": null, "evidence": [{"asset": "source:pmid:31616474", "locator": "sections[18] \"Data Availability\" paragraph 2", "quote": "The datasets generated for this study are available on request to the corresponding author."}], "state": "complete"}], "gaps": []}, "experiment": {"state": "partial", "objective": null, "steps": [{"id": "read_published_estimate", "order": 1, "name": "Read the published estimate from the source", "kind": "other", "state": "complete", "blocked_on": null, "description": "The claim, method and evidence span published with the source article.", "duration_hours": null, "parameters": {}, "inputs": ["source:pmid:31616474"], "outputs": ["the published estimate and its evidence"], "instruments": [], "reagents": []}, {"id": "obtain_source_data", "order": 2, "name": "Obtain the source data from the named repository", "kind": "data-preparation", "state": "blocked", "blocked_on": "data:accession_absent", "description": "The GEO series accession resolves and its sample accessions are listed. This lane did not download the dataset or read its licence.", "duration_hours": null, "parameters": {}, "inputs": [], "outputs": ["the source data with its repository provenance"], "instruments": [], "reagents": []}, {"id": "run_analysis", "order": 3, "name": "Run the published analysis", "kind": "analysis", "state": "complete", "blocked_on": null, "description": "Apply the paper's statistical method to the resolved series and compare the result with the published target.", "duration_hours": null, "parameters": {}, "inputs": [], "outputs": ["the replicated result and its comparison"], "instruments": [], "reagents": []}], "materials": [], "instruments": [], "reagents": [], "conditions": {}, "gaps": ["step:obtain_source_data blocked on data:accession_absent"]}, "data": {"state": "partial", "assets": [{"id": "source:pmid:31616474", "role": "manuscript", "format": "xml", "media_type": "application/xml", "uri": "https://doi.org/10.3389/fgene.2019.00884", "sha256": "sha256:b87909c971ff44c51732d0c3548302ba8a3430c5ef8a42ecef2341a77a4826db", "bytes": 50143, "access": "open", "state": "resolved", "reader": null, "reader_status": "reader_required", "identifier": "10.3389/fgene.2019.00884", "version": null, "note": "Full article fetched from PMC; the checksum covers the rendered section body, not the original XML bytes.", "structure": {"pmcid": "PMC6763691", "source": "europe_pmc_fulltext", "coverage": "whole_body", "text_hash_scope": "rendered_section_body"}}, {"id": "asset:table:pmid:31616474:1", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:415074d77153faad007696ca352403c85eb19bc1ce902fc2542c648e77764706", "bytes": 2088, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[5]/table-wrap[1]#T1", "version": null, "note": "Differential MuD expression and survival correlation revealed by UALCAN and GEPIA.", "structure": {"pmcid": "PMC6763691", "section_index": 5, "section_title": "Sample Collection From Human Brain Tumors", "row_count": 33, "column_count": 8}, "table": {"label": "Table 1", "caption": "Differential MuD expression and survival correlation revealed by UALCAN and GEPIA.", "rows": [["", "Project name", "UALCAN", "GEPIA", "GR(U)", "GRG)", "S (U)", "S (G)"], ["BRCA", "Breast invasive carcinoma", "N (n = 114)", "N (n = 291)", "NS", "NS", "C", "No"], ["T (n = 1094)", "T (n = 1085)", "(HL)"], ["CHOL", "Cholengiocarcinoma", "N (n = 9)", "N (n = 9)", "Up", "NS", "No", "No"], ["T (n = 36)", "T (n = 36)"], ["COAD", "Colon adenocarcinoma", "N (n = 41)", "N (n = 349)", "Down", "NS", "No", "No"], ["T (n = 286)", "T (n = 275)"], ["DLBC", "Lymphoid neoplasm diffuse large B-cell lymphoma", "–", "N (n = 337)", "–", "Up", "No", "No"], ["T (n = 47)"], ["ESCA", "Esophageal carcinoma", "N (n = 11)", "N (n = 286)", "Up", "Up", "No", "No"], ["T (n = 184)", "T (n = 182)"], ["GBM", "Glioblastoma multiforme", "N (n = 5)", "N (n = 207)", "–", "Up", "No", "No"], ["T (n = 156)", "T (n = 163)"], ["KICH", "Kidney chromophobe", "N (n = 25)", "N (n = 53)", "NS", "Up", "No", "No"], ["T (n = 67)", "T (n = 66)"], ["KIRC", "Kidney renal clear cell carcinoma", "N (n = 72)", "N (n = 100)", "Down", "Down", ".C", "C"], ["T (n = 533)", "T (n = 523)", "(HH)", "(HH)"], ["KIRP", "Kidney renal papillary cell carcinoma", "N (n = 32)", "N (n = 60)", "Down", "NS", "No", "No"], ["T (n = 290)", "T (n = 286)"], ["LGG", "Lower-grade glioma", "N (n = 248)", "N (n = 207)", "NS", "Up", "No", "No"], ["T (n = 265)", "T (n = 518)"], ["LUAD", "Lung adenocarcinoma", "N (n = 29)", "N (n = 347)", "NS", "NS", "C", "No"], ["T (n = 519)", "T (n = 483)", "(HL)"], ["OV", "Ovarian serous cystadenocarcinoma", "–", "N (n = 88)", "–", "Up", "No", "No"], ["T (n = 426)"], ["PAAD", "Pancreatic Adenocarcinoma", "N (n = 4)", "N (n = 171)", "NS", "Up", "No", "No"], ["T (n = 178)", "T (n = 179)"], ["READ", "Rectum adenocarcinoma", "N (n = 11)", "N (n = 92)", "Down", "NS", "No", "No"], ["T (n = 166)", "T (n = 318)"], ["STAD", "Stomach adenocarcinoma", "N (n = 34)", "N (n = 34)", "NS", "Up", "No", "No"], ["T (n = 415)", "T (n = 415)"], ["THYM", "Thymoma", "N (n = 2)", "N (n = 339)", "NS", "Up", "No", "No"], ["T (n = 120)", "T (n = 118)"]], "row_count": 33, "column_count": 8, "numeric_cells": 60, "header_rows": 1, "locator": "section[5]/table-wrap[1]#T1"}}, {"id": "asset:table:pmid:31616474:2", "role": "table", "format": "jats-table", "media_type": "application/vnd.jats.table+json", "uri": null, "sha256": "sha256:3dac197bb1793d5d0337b1e9ff78511cf48fbccc7730e54311fdb4006982a6b0", "bytes": 1272, "access": "open", "state": "verified", "reader": "read_table", "reader_status": "available", "identifier": "section[16]/table-wrap[1]#T2", "version": null, "note": "Clinical characteristics of patients with brain tumor in this study. GB, glioblastoma; ODG, oligodendroglioma; ED, ependymoma.", "structure": {"pmcid": "PMC6763691", "section_index": 16, "section_title": "Expression Analysis Revealed Putative Post-Translational Regulation of MuD", "row_count": 13, "column_count": 9}, "table": {"label": "Table 2", "caption": "Clinical characteristics of patients with brain tumor in this study. 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