LAMC1 aggravates diabetic retinopathy through PI3K/AKT signaling-regulated epithelial-mesenchymal transition in retinal pigment epithelial cells.

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By analyzing DR-related microarray datasets (GSE60436/GSE102485) from GEO, we identified 685 differentially expressed genes (570 downregulated, 115 upregulated). Functional and WGCNA analyses linked these to PI3K/Akt signaling, revealing 11 diagnostic hub genes, including LAMC1. Western blot analysis confirmed that LAMC1 significantly upregulated in high glucose (HG)-treated ARPE-19 cells and diabetic mouse retinas. In vitro and in vivo experiments confirmed that LAMC1 promotes EMT in retinal pigment epithelial (RPE) cells via PI3K/Akt activation, enhancing migration and invasion. Conversely, LAMC1 knockdown alleviated retinal damage in diabetic mice. 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By analyzing DR-related microarray datasets (GSE60436/GSE102485) from GEO, we i"], "order_basis": "paper_text_order:269", "id": "assay:1"}, {"role": "data_generation", "definition_id": "western_blot", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["ng 11 diagnostic hub genes, including LAMC1. Western blot analysis confirmed that LAMC1 significantly", "apidly frozen at - 80°C and was reserved for Western blot analysis. Statistical analysis All statistic"], "order_basis": "paper_text_order:524", "id": "assay:2"}, {"role": "data_generation", "definition_id": "animal_model", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["lls and diabetic mouse retinas. In vitro and in vivo experiments confirmed that LAMC1 promotes EM", "cose (HG)-treated ARPE-19 cells and diabetic mouse retinas. In vitro and in vivo experiments co", "wing a 7-day period of adaptive feeding, the mice were randomly allocated into 4 distinct groups, e", "ecutive days. In contrast, the Control group mice were injected with an equivalent volume of citrat"], "order_basis": "paper_text_order:649", "id": "assay:3"}, {"role": "data_generation", "definition_id": "transfection", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.85, "evidence": ["ng migration and invasion. Conversely, LAMC1 knockdown alleviated retinal damage in diabetic mice."], "order_basis": "paper_text_order:850", "id": "assay:4"}, {"role": "data_generation", "definition_id": "cell_culture", "definition_version": "99e1195c7d6f", "definition_digest": null, "required_operations": [], "support_state": "partial", "source_hashes": {"assay_lexicon": "99e1195c7d6f"}, "confidence": 0.99, "evidence": ["% penicillin/streptomycin (from Gibco). The cell culture was maintained in a humidified incubator set at a temperatu", "ocated in Shanghai, China). 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